Biodegradation of cassava starch factory residue using a combination of cellulases.pdf

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Biodegradation of cassava starch factory residue using a combination of cellulases.pdf

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Biodegradation of cassava starch factory residue using a combination of cellulases

b i om a s s a n d b i o e n e r g y 3 5 ( 2 0 1 1 ) 1 2 1 1e1 2 1 8Avai lab le a t www.sc iencedi rec t .com ht tp : / /www.e lsev ier . com/ loca te /b iombioeBiodegradation of cassava starch factory residue using a combination of cellulases, xylanases and hemicellulasesM.P. Divya Nair, G. Padmaja*, S.N. Moorthy Division of Crop Utilization, Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695 017, Kerala, Indiaa r t i c l e i n f o Article history: Received 11 October 2010 Received in revised form 3 December 2010 Accepted 7 December 2010 Available online 5 January 2011 Keywords: Cassava starch factory residue Biodegradation Cellulase Hemicellulase Xylanase Glucose release* Corresponding author. Tel.: t91 471 259855 E-mail address: padmajabn@ 0961-9534/$ e see front matter a 2010 Elsev doi:10.1016/j.biombioe.2010.12.009a b s t r a c t Cassava starch factory residue (CSFR), a low cost solid byproduct of the cassava starch industry, having around 60% unextracted starch and 15% cellulose is a potential candidate for bioethanol production. As the economic feasibility depends on complete degradation of the polysaccharides to fermentable glucose, the comparative hydrolytic potential of four enzymes such as Multifect XL, Optimash XL, Optimash BG and Accellerase1000 was studied. Whilst the former three enzymes exerted maximum activity at pH 5.0 and 60 C, Accellerase had optimum activity at pH 4.5 and 60 C. The enzyme cocktail, Multifect XL- Optimash XL-Accellerase (5, 15 and 20 g enzyme protein kg1 CSFR respectively) was more effective than sequential process with the same enzyme loading. Hydrothermal treatment (HT) of CSFR for 30 min at 100 C enhanced the susceptibility to enzymatic cleavage as compared to HT for 45 and 60 min as well as autoclaving or microwave exposure. Optimash BG was the most effective on HT CSFR and approximately 704.8 g glucose was released kg1 CSFR. The high yield of glucose indicates the potential use of hydrotherm