Characterization and properties of catalase immobilized onto controlled pore glass and its applicati.pdf

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Characterization and properties of catalase immobilized onto controlled pore glass and its applicati.pdf

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Characterization and properties of catalase immobilized onto controlled pore glass and its applicati

C g ? U a A R R 1 A A K C C C R P 1 e m t ( i u l a g i c ( a 1 dJournal of Molecular Catalysis B: Enzymatic 58 (2009) 124–131 Contents lists available at ScienceDirect Journal of Molecular Catalysis B: Enzymatic journa l homepage: www.e lsev ier .com/ locate /molcatb haracterization and properties of catalase immobilized onto controlled pore lass and its application in batch and plug-flow type reactors zlem Alptekin ?, S. Seyhan Tükel, Deniz Y?ld?r?m, Dilek Alag?z niversity of Cukurova, Faculty of Arts Sciences, Department of Chemistry, 01330 Adana, Turkey r t i c l e i n f o rticle history: eceived 18 July 2008 eceived in revised form 3 November 2008 ccepted 9 December 2008 vailable online 24 December 2008 a b s t r a c t Bovine liver catalase was covalently immobilized onto controlled pore glass (CPG) beads modified with 3-aminopropyltriethoxysilane (3-APTES) followed by treatment with glutaraldehyde. Coupling of cata- lase onto CPG was optimized to improve the efficiency of the overall immobilization procedure. The optimum coupling conditions: pore diameter of CPG, pH, buffer concentration, temperature, coupling time and initial catalase amount per grams of carrier were determined as 70nm, 6.0, 75mM, 5 ?C, 7heywords: atalase ontrolled pore glass ovalent immobilization and 6mg catalase, respectively. Catalytic efficiencies (kcat/Km) and thermal inactivation rate constants (ki) of ICPG1 were determined and compared with that of free catalase. Suitability of ICPG1 was also investigated by using it in batch and plug-flow type reactors. When the remaining activity of ICPG1 retained was about 50% of its initial activity the highest total productivity of ICPG1 was determined as 7.6×106 Ug immobilized catalase?1 in plug-flow type reactor. However, the highest total productivity of 5 mob ?1 tion eactors roductivity ICPG1 was 6.2×10 Ug im biocatalyst for the applica . Introduction Catalases (H2O2:H2O2 oxidoreductase; EC ) are abundant nzymes in nature that decompose hydr