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Chitosan and mint mixture A new preservative for meat and meat products
Available online at /locate/foodchem
Food Chemistry 107 (2008) 845–852
Food
ChemistryChitosan and mint mixture: A new preservative
for meat and meat products
Sweetie R. Kanatt, Ramesh Chander *, Arun Sharma
Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India
Received 29 June 2007; received in revised form 13 August 2007; accepted 31 August 2007Abstract
Meat is prone to both microbial and oxidative spoilage and therefore it is desirable to use a preservative with both antioxidant and
antimicrobial properties. Mint extract alone had good antioxidant activity but poor antimicrobial activity, while chitosan alone showed
poor antioxidant activity with excellent antimicrobial properties. Therefore, the potential of chitosan and mint mixture (CM), as a
preservative for meat and meat products, was investigated. Addition of chitosan to mint extract did not interfere with the antioxidant
activity of mint. In the case of 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, the IC50 value for CM (17.8 lg/ml) was significantly
(p 6 0.05) lower than that for mint extract (23.6 lg/ml). CM efficiently scavenged superoxide and hydroxyl radicals. The antimicrobial
activities of CM and chitosan were comparable against the common food spoilage and pathogenic bacteria, the minimum inhibitory
concentration being 0.05%. CM was more effective against Gram-positive bacteria. The shelf life of pork cocktail salami, as determined
by total bacterial count and oxidative rancidity, was enhanced in CM-treated samples stored at 0–3 C.
2007 Elsevier Ltd. All rights reserved.
Keywords: Chitosan mint mixture; Antioxidant; Antimicrobial; Meat products1. Introduction
Chitosan is a versatile biopolymer, having a broad range
of applications in the food industry (Rudrapatnam Far-
ooqahmed, 2003). It exhibits antimicrobial activity against
a range of food-borne microorganisms and consequently
has attracted attention as a potential natural food preser-
vative (Chen, Lian, Isa
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