Denitive identication of louping ill virus by RT-PCR and sequencing in eld populations of I.pdf
- 1、本文档共11页,可阅读全部内容。
- 2、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
Denitive identication of louping ill virus by RT-PCR and sequencing in eld populations of I
Arch Virol (1997) 142: 1181±1191
De?nitive identi?cation of louping ill virus by RT-PCR
and sequencing in ?eld populations of Ixodes ricinus
on the Lochindorb Estate
M. W. Gaunt1, L. D. Jones1, K. Laurenson2, P. J. Hudson2, H. W. Reid3,
and E. A. Gould1
1NERC, Institute of Virology and Environmental Microbiology, Oxford, U.K.
2 Wildlife Epidemiology Unit, Department of Biological and
Molecular Science, University of Stirling, Stirling, U.K.
3 Moredun Research Institute, Edinburgh, U.K.
Accepted January 6, 1997
Summary. Rapid and precise virus detection procedures are an important
component of any epizootiological study. An automated one tube reverse
transcriptase and nested primer polymerase chain reaction (RT-PCR) followed
by nucleotide sequencing of the cDNA product, was used for the rapid detection
and identi?cation of louping ill (LI) virus in ?eld caught Ixodes ricinus and
compared with a classical isolation method i.e. infectivity in cell culture. The
results establish the genetic identity of LI virus on the Lochindorb Estate. There
was a high correlation between the results obtained by RT-PCR and infectivity
assays. RT-PCR and sequencing proved to be a rapid and accurate system for
identifying LI virus in ?eld specimens. Development of this system should
improve the capacity to undertake detailed epizootiological studies of LI virus.
Introduction
Louping ill (LI) is a debilitating virus disease producing high rates of mortality
in infected grouse [21, 23]. This is especially a problem today in the Scottish
highlands where grouse estates form an integral part of the local economy. In
mainland Britain, LI virus occurs speci?cally in areas of upland sheep grazing,
which include Scotland and the North Yorkshire moors and is transmitted by
the 3-host ixodid tick species Ixodes ricinus [22]. Until recently sheep were the
only species regarded as essential for the transmission and persistence of LI
virus. However, recent studies have demonstrated the ef?cient transm
您可能关注的文档
- chemical priming with urea and KNO3 enhances maiz hybids seed viability under abiotic stress.pdf
- chemical safety of meat products.pdf
- CHATTERBOX BOOK 4(1-2单元).pdf
- Chemical characterization and source apportionment of PM2.5.pdf
- Chemically modified light-curable chitosans with enhanced.pdf
- Chest Ultrasonography in the ICU.pdf
- Chi CVPR 2013 - Block and Group Regularized Sparse Modeling for Dictionary Learning.pdf
- Chest study PPA.pdf
- Chiffres clés Vente à distance e - commerce fournie par FEVAD.pdf
- Childhood Maltreatment and Conduct DisorderIndependent Predictors of Adolescent Substance among heal.pdf
最近下载
- 2024-2030年中国六氟磷酸锂行业市场现状供需分析及投资评估规划分析研究报告.docx
- 防护工程之三维网植被护坡(附施工图解、工艺、造价编制).pdf VIP
- 《中铁一局集团有限公司工程项目管理绩效考核办法》(中铁一成本[2015]623号).doc
- 机械打眼开炸石方(附施工图解、造价编制).pdf VIP
- 路基土石方(借土填方)(附施工图解、工艺、造价编制).pdf VIP
- 2023扬州龙川控股集团有限责任公司招聘试题及答案解析.docx
- 交通安全设施之交通标志(附施工图解、工艺、造价编制).pdf VIP
- 路基土石方之填挖交界(附施工图解、工艺、造价编制).pdf VIP
- 2纵横造价实战--抛石挤淤附施工图解、工艺、造价编制.pdf
- 2024《我的阿勒泰》作品简介PPT课件(精品).pptx
文档评论(0)