Effect of dietary protein reduction with lysine and methionnine supplementation on growth grass carp.pdf

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2017-04-09 发布于江苏
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Effect of dietary protein reduction with lysine and methionnine supplementation on growth grass carp.pdf

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Effect of dietary protein reduction with lysine and methionnine supplementation on growth grass carp

1,2 1 1 1 1 1 1 1 1 School of Life Science, Sun Yat-Sen University, Guangzhou, China; 2 Animal Science College, South China Agricultural University, Guangzhou, China A 63-day growth trial was undertaken to estimate the effects of supplemented lysine and methionine with differ- ent dietary protein levels on growth performance and feed utilization in Grass Carp (Ctenopharyngodon idella). Six plant-based practical diets were prepared, and 32CP, 30CP and 28CP diets were formulated to contain 320 g kg1, 300 g kg1 and 280 g kg1 crude protein without lysine and methionine supplementation. In the supplementary group, lysine and methionine were added to formulate 32AA, 30AA and 28AA diets with 320 g kg1, 300 g kg1 and 280 g kg1 dietary crude protein, respectively, accord- ing to the whole body amino acid composition of Grass Carp. In the groups without lysine and methionine supple- mentation, weight gain (WG, %) and specific growth rate (SGR, % day1) of the fish fed 32CP diet were significantly higher than that of fish fed 30CP and 28CP diets, but no significant differences were found between 30CP- and 28CP-diet treatments. WG and SGR of the fish fed 32AA and 30AA diets were significantly higher than that of fish fed 28AA diets, and the performance of grass carp was also significantly improved when fed diets with lysine and methionine supplementation (P 0.05), and the interaction between dietary protein level and amino acid supplementa- tion was noted between WG and SGR (P 0.05). Feed intake (FI) was significantly increased with the increase in dietary protein level and the supplementation of lysine and methionine (P 0.05), but feed conversion ratio (FCR) showed a significant decreasing trend (P 0.05). Two days after total ammonia nitrogen (TAN) concentration test, the values of TAN discharged by the fish 8 h after feeding were 207.1, 187.5, 170.6, 157.3, 141.3 and 128.9 mg kg1 body weight for fish fed 32CP, 32AA, 30CP, 30AA, 28CP and 28AA diets, respectively. TA