Epidermal and Dermal Characteristics in Skin Equivalent....pdf

Epidermal and Dermal Characteristics in Skin Equivalent after Systemic and Topical Application of Skin Care Ingredients JANA VICANOVA,a CHARBEL BOUEZ,b SOPHIE LACROIX,b LARS LINDMARK,c AND ODILE DAMOURb aDermData s.r.o, Czech Republic bBanque de Tissus et Cellules, Ho?pital Edouard Herriot, Hospices Civils de Lyon, Lyon, France cImedeen Research, Ferrosan, Denmark ABSTRACT: Effects of active ingredients from topical and systemic skin- care products on structure and organization of epidermis, dermal– epidermal junction (DEJ), and dermis were examined using an in vitro reconstructed skin equivalent (SE). Imedeen Time Perfection (ITP) in- gredients (a mixture of BioMarine Complex, grape seed extract, tomato extract, vitamin C) were supplemented systemically into culture medium. Kinetin, an active ingredient from Imedeen Expression Line Control Serum, was applied topically. Both treatments were tested separately or combined. In epidermis, all treatments stimulated keratinocyte prolifer- ation, showing a significant increase of Ki67-positive keratinocytes (P 0.05). Kinetin showed a twofold increase of Ki67-positive cells, ITP re- sulted in a fivefold, and ITP+kinetin showed a nine-fold increase. Differ- entiation of keratinocytes was influenced only by kinetin since filaggrin was found only in kinetin and kinetin+ITP samples. At the DEJ, laminin 5 was slightly increased by all treatments. In dermis, only ITP increased the amount of collagen type I. Both kinetin and ITP stimulated forma- tion of fibrillin-1 and elastin deposition. The effect of kinetin was seen in upper dermis. It stimulated not only the amount of deposited fibrillin-1 and elastin fibers but also their organization perpendicularly to the DEJ. ITP stimulated formation of fibrillin-1 in deeper dermis. In summary, the combination of topical treatment with kinetin and systemic treat- ment with ITP had complementary beneficial effects in the formation and development of epidermis and dermis. KEYWORDS: Imedeen