Epidermal differentiation governs engineered skin biomechanics.pdf

下载文档

6
0
约3.95万字
约 8页
2017-04-09 发布于江苏
举报
版权申诉
保障服务

Epidermal differentiation governs engineered skin biomechanics.pdf

1、本文档共8页，可阅读全部内容。
2、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

Epidermal differentiation governs engineered skin biomechanics

Journal of Biomechanics 43 (2010) 3183–3190Contents lists available at ScienceDirectjournal homepage: /locate/jbiomech Journal of Biomechanics0021-92 doi:10.1 n Corr The Oh 43210, E-mwww.JBEpidermal differentiation governs engineered skin biomechanicsG.C. Ebersole a, P.M. Anderson a, H.M. Powell a,b,n a Department of Materials Science and Engineering, The Ohio State University, 116 W. 19th Ave., 243C Fontana Labs, Columbus, OH 43210, USA b Department of Biomedical Engineering, The Ohio State University, The Ohio State University, 116 W. 19th Ave., 243C Fontana Labs, Columbus, OH 43210, USAa r t i c l e i n f o Article history:Accepted 24 July 2010 Engineered skin must be mechanically strong to facilitate surgical application and prevent damage during the early stages of engraftment. However, the evolution of structural properties during culture,Keywords: Tissue engineering Skin Differentiation Strength90/$ - see front matter 2010 Elsevier Ltd. A 016/j.jbiomech.2010.07.026 esponding author at: Department of Materi io State University, 116 W. 19th Ave., 243C USA. Tel.: +1 614 247 8673; fax: +1 614 292 ail address: powell.299@ (H.M. Powela b s t r a c t the relative contributions of the epidermis and dermis, and any correlation with tissue morphogenesis are not well known. These aspects are investigated by assessing the mechanical properties of engineered skin (ES) and engineered dermis (ED) during a 21-day culture period, including correlations with cellular metabolism, cellular organization and epidermal differentiation. During culture, the epidermis differentiates and begins to cornify, as evidenced by immunostaining and surface electrical capacitance. Tensile testing reveals that the ultimate tensile strength and linear stiffness increase linearly with time for ES, but are relatively unchanged for ED. ES strength correlates significantly with epidermal differentiation (po0.001) and a composite strength model indicates that strength is largely determined by the epi