of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass.pdf
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of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass
University of Nebraska - Lincoln
DigitalCommons@University of Nebraska - Lincoln
Publications from USDA-ARS / UNL Faculty USDA Agricultural Research Service --Lincoln,Nebraska
1-1-2009
Visualization of Biomass Solubilization and
Cellulose Regeneration During Ionic Liquid
Pretreatment of Switchgrass
Seema Singh
Joint BioEnergy Institute
Blake A. Simmons
Joint BioEnergy Institute
Kenneth P. Vogel
University of Nebraska-Lincoln
Follow this and additional works at: /usdaarsfacpub
Part of the Agricultural Science Commons
This Article is brought to you for free and open access by the USDA Agricultural Research Service --Lincoln, Nebraska at DigitalCommons@University
of Nebraska - Lincoln. It has been accepted for inclusion in Publications from USDA-ARS / UNL Faculty by an authorized administrator of
DigitalCommons@University of Nebraska - Lincoln.
Singh, Seema; Simmons, Blake A.; and Vogel, Kenneth P., Visualization of Biomass Solubilization and Cellulose Regeneration During
Ionic Liquid Pretreatment of Switchgrass (2009). Publications from USDA-ARS / UNL Faculty. Paper 395.
/usdaarsfacpub/395
ARTICLE
Visualization of Biomass Solubilization and
Cellulose Regeneration During Ionic Liquid
Pretreatment of Switchgrass
Seema Singh,1,2 Blake A. Simmons,1,2 Kenneth P. Vogel3
1Joint BioEnergy Institute, Deconstruction Division, Emeryville, California;
telephone: 925-337-6154; fax: 925-294-3020; e-mail: basimmo@
2Sandia National Laboratories, Biomass Science and Conversion Technology Department,
Livermore, California
3United States Department of Agriculture, Grain, Forage and Bioenergy Research Unit,
USDA-ARS, University of Nebraska, Lincoln, Nebraska
Received 4 March 2009; revision received 17 April 2009; accepted 27 April 2009
Published online 4 May 2009 in Wiley InterScience (). DOI 10.1002/bit.22386
ABSTRACT: Auto-fluorescent mapping of plant cell walls
was used to visualize cellulose and lignin in pristine
switchgrass (Panicum virgatum) stems to determine the
mechanisms of bi
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