Southern, Northern and Western blotting.pdf

Southern, Northern and Western blotting 生理所 黃阿敏 生物秀—专心做生物！ . i . 易生物--领先的生物医药商务平台 . i . 生物秀论坛--学术交流,,资源共享,,互助社区 . i . //bbs// 生物秀实验频道——生命科学实验中心 /bio101 生物秀论坛——学术交流、资源共享、互助社区 /bbs Comparison of Southern, Northern, and Western analyses of Gene X 生物秀实验频道——生命科学实验中心 /bio101 生物秀论坛——学术交流、资源共享、互助社区 /bbs Southern hybridization n First described by E. M. Southern in 1975. n Applications of Southern hybridization n RFLP’s, VNTR’s and DNA fingerprinting n Checking of the gene knockout mice n The flow chart of Southern hybridization 生物秀实验频道——生命科学实验中心 /bio101 生物秀论坛——学术交流、资源共享、互助社区 /bbs Southern hybridization Transfer buffer 生物秀实验频道——生命科学实验中心 /bio101 生物秀论坛——学术交流、资源共享、互助社区 /bbs Detection of an RFLP by Southern blotting 生物秀实验频道——生命科学实验中心 /bio101 生物秀论坛——学术交流、资源共享、互助社区 /bbs Detection of the sickle-cell globin gene by Southern blotting 生物秀实验频道——生命科学实验中心 /bio101 生物秀论坛——学术交流、资源共享、互助社区 /bbs More than 3000 human genetic diseases are attributable to single-gene defects. In most of these the mutation is recessive: that is, it shows its effect only when an individual inherits two defective copies of the gene, one from each parent. One goal of modern medicine is to identify those fetuses that carry two copies of the defective gene long before birth so that the mother, if she wishes, can have the pregnancy terminated. In sickle-cell anemia, for example, the exact nucleotide change in the mutant gene is known (the sequence GAG is changed to GTG at a specific point in the DNA strand that codes for the β chain of hemoglobin). For prenatal diagnosis, two DNA oligonucleotides are synthesized - one c