小麦Puroindoline b-2基因等位变异及其对产量性状的影响.doc

小麦Puroindoline b-2基因变异与产量相关性状的分析 河南农业大学农学院 / 河南省粮食作物协同创新中心, 河南郑州 450002 陈锋 李向楠 曹莹莹 孙建喜 张福彦 董中东 崔党群 摘要：Puroindoline b-2（Pinb-2）是近期发现的一组与Puoindoline b基因具有高度相似性的基因。先前研究已经表明，Pinb-2基因在普通小麦B基因组中存在有多种变异类型，软质麦中不同变异类型间籽粒硬度存在着显著差异。本研究在此基础上进一步分析了普通小麦B基因组中的Pinb-2基因等位变异与产量相关性状以及旗叶大小的关系，并利用重组近交系群体进行了染色体定位。结果表明，4种不同变异类型的材料中，拥有Pinb-2v3b类型的小麦品种千粒重、籽粒直径、穗粒数、穗粒重和单株粒重均为最高，表明拥有Pinb-2v3b类型的小麦品种产量相关性状可能优于其它3种类型。另外，拥有Pinb-2v3a类型的小麦品种旗叶宽度、旗叶长度和旗叶面积均高于其它3种类型。采用SSR标记定位结果表明，Pinb-2v2和Pinb-2v3互为一对等位基因，位于7B染色体的长臂上，与标记Barc315连锁。本研究为进一步研究Puroindoline b-2基因的分子遗传基础提供了重要的理论依据，同时也为我国高产小麦育种提供了有用信息。 关键词：普通小麦；Puroindoline b-2基因；产量相关性状；旗叶大小；基因定位 Analysis of association of Puroindoline b-2 alleles with yield-related traits in bread wheat (Triticum aestivum L.) CHEN Feng, LI Xiang-Nan, Cao Ying-Ying, Sun Jianxi, Zhang Fu-Yan, Dong Zhong-Dong, Cui Dang-Qun Agronomy College / Collaborative Innovation Center of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Abstract: Puroindoline b-2 (Pinb-2) genes were recently discovered and possess high similarity with Puroindoline b gene. In our previous study, several Pinb-2 alleles have been identified in B genome of bread wheat and allelic variation of Pinb-2 was associated with grain texture in soft wheat. In this study, we further analyzed the association of Pinb-2 alleles with yield-related traits and flag leaf size as well as genetic mapping of Pinb-2 in bread wheat. Results indicated that cultivars with Pinb-2v3b possessed the highest 1000-kernel weight, grain diameter, grain number per spike, grain weight per spike and grain weight per plant amongst wheat cultivars with four of Pinb-2v2, Pinb-2v3a, Pinb-2v3b and Pinb-2v3c. Therefore, a conclusion could be drawn that cultivars with Pinb-2v3b possibly have more preferable yield-related traits than those of others. Additionally, cultivars with Pinb-2v3a allele possessed the highest value of width, length and area of flag leaf among wheat genotypes with four Pinb-2 alleles. Geneti