Antioxidant, anti-hyaluronidase and antifungal activities of Melaleuca leucadendron Linn. leaf oils.pdf

ORIGINAL ARTICLE Antioxidant, anti-hyaluronidase and antifungal activities of Melaleuca leucadendron Linn. leaf oils Pujiarti Rini ? Yoshito Ohtani ? Hideaki Ichiura Received: 16 November 2011 / Accepted: 14 May 2012 / Published online: 5 June 2012  The Japan Wood Research Society 2012 Abstract The present study examined the chemical com- position, in vitro antioxidant, anti-hyaluronidase and anti- fungal activities of essential oils of Melaleuca leucadendron Linn. from Gundih-Central Java, Indonesia in different plant ages of 5, 10 and 15 years old. The Chemical composition of essential oils were analyzed by GC/MS. Twenty-six compo- nents were identified, of which 1,8-cineole (49.22–55.04 %), a-terpineol (8.79–10.70 %), d-limonene (5.58–6.39 %), and b-caryophyllene (5.03–7.64 %) were the main compounds in these oils. The antioxidant assay and anti-hyaluronidase assay showed that M. leucadendron leaf oils possess mild antioxi- dant activity with IC50 between 7.21 and 9.23 mg/ml and anti- hyaluronidase activity with IC50 between 1.94 and 3.03 mg/ ml. The antifungal assay showed the effectiveness of these essential oils against Fomitopsis palustris (IC50 0.12– 3.16 mg/ml), Trametes versicolor (IC50 0.01–0.06 mg/ml), Cladosporium cladosporioides (IC50 0.03–0.49 mg/ml), and Chaetomium globosum (IC50 0.06–0.15 mg/ml). Keywords Melaleuca leucadendron Linn.  Essential oil  Antioxidant  Anti-hyaluronidase  Antifungal Introduction Essential oils and their components have recently been of great interest because of their relatively safe status, wide acceptance by consumer and the possibility of their exploitation for potential multi-purpose functional uses [1]. Many researchers have reported utilization of Melaleuca essential oils, mainly used in the manufacture of cosmetics, germicides, as clinical efficacy and medicinal. These essential oils are also used as antiseptic, antibacterial, antiviral, antifungal, antiprotozoal, antioxidant, anti- inflammatory agents and oral