Effect of cytokinins on shoot regeneration from cotyledon and leaf segment.pdf

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Effect of cytokinins on shoot regeneration from cotyledon and leaf segment.pdf

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Effect of cytokinins on shoot regeneration from cotyledon and leaf segment

Research Note Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai) De-Ping Guo1, Zhu-Jun Zhu1, Xiao-Xiang Hu1 Si-Jun Zheng1,2,* 1Department of Horticulture, Zhejiang University 310029, Hangzhou, P.R. China; 2Plant Research International, Wageningen University and Research Centre, P.O. Box 16, 6700AA, Wageningen, The Netherlands (*requests for offprints: Lab. of Entomology, Wageningen University, 6700 EH, The Netherlands; Phone: +31 317 482328; Fax: +31 317 484821; E-mail: sijun.zheng@wur.nl) Received 24 November 2003; accepted in revised form 14 March 2005 Key words: Brassica juncea var. tsatsai, cytokinins, stem mustard, tissue culture Abstract Cotyledon and leaf segments of stem mustard (Brassica juncea var. tsatsai) were cultured on Murashige and Skoog medium supplemented with various concentrations of different cytokinins [6-benzyladenine (BA), N-(2-chloro-4-pyridyl)-n-phenylurea (CPPU), 6-furfurylaminopurine (KT) and thidiazuron (TDZ)] in combinations with different levels of a-naphthalene acetic acid (NAA). The shoot regeneration frequency of cotyledon and leaf segment was dependent on the kinds and concentrations of cytokinins used in the medium, while in most cases cotyledon gave high regeneration frequency than leaf segment. TDZ proved to be the best cytokinin to induce shoot from both cotyledon and leaf segments compared to BA, KT and CPPU. The highest frequency of shoot regeneration was 61.3–67.9 % in cotyledon and 40.7–52.4% in leaf segment respectively when 2.27 or 4.54 lM TDZ was combined with 5.37 lM NAA. Next to TDZ, CPPU was also very suitable to induce shoot formation both in cotyledon and leaf segment. When 1.61 lMCPPU was combined with 2.69 lM NAA, shoot regeneration frequency was 45.0% in cotyledon and 36.4% in leaf segment, respectively. It was also shown that KT and BA affected shoot regeneration from cotyledon and leaf segment, the shoot regeneration was greatly increased w