Retroviruses逆轉录病毒.pptVIP

Retroviruses逆转录病毒 Yihong PENG Associate professor Dept of Microbiology Health Science Center Peking University (04-03-11) HIV and AIDS HIV and AIDS AIDS Statistics AIDS Statistics AIDS Statistics AIDS Statistics AIDS Statistics HIV and AIDSThe Virus The Genome of HIV The Genome of HIV HIV - The Virus HIV - The Virus HIV - The Virus TYPE HIV-1(evn) Subtype M: A---J O: 1 N: 1 HIV-2 Subtype A-F HIV - Life History HIV - Life History HIV - Life History Why do all T4 cells disappear? - 2 Inducing T cell apoptosis HIV and AIDS HIV and AIDS HIV and AIDS Other cells infected by HIVCD4- HIV and AIDSan infectious agent Anti-HIV Strategies Anti-HIV Strategies HIV的结构基因和蛋白质表达 HIV的结构基因和蛋白质表达 HIV的结构基因和蛋白质表达 HIV的结构基因和蛋白质表达 HIV的结构基因和蛋白质表达  Cytotoxic T cell Killing of CD4 cells3. Cytotoxic T cell-mediated lysis Why do all T4 cells disappear? BUT: Most cells are not infected The course of the disease 原发感染急性期 潜伏期 AIDS相关综合征 AIDS The cellular and immunological picture - The course of the disease Viral load predicts survival time Epithelial cells Endothelial cells of brain Brains cells : Astroglia, oligodendroglia Galactocerebroside receptor (半乳糖脑苷脂) 原虫 卡氏肺囊虫－ Pneumocystis carinii pneumonia， 弓形体病 细菌（鸟-胞内结核分枝杆菌、星形放线菌） 病毒（CMV、HSV、HBV、VZV、HHV-8等） 真菌（白色念珠菌、新隐球菌等） Opportunity Infection and Cancer 1979 - 5 cases of Pneumocystis carinii With giemsa stain at high magnification, the faint bluish dot-like intracystic bodies of Pneumocystis carinii in lung are seen in this cytologic preparation from a bronchoalveolar lavage In Los Angeles 1967-1978 only two cases of Pneumocystis carinii pneumonia All Homosexual 毛白舌 Kaposi肉瘤 1.检测抗体：ELISA, RIP, IFA, Western blot 筛查试验: ELISA 确证试验: Western blot; 查到2种抗体如gp120和p24抗体方可确诊 2. 病毒p24抗原检测:急性期和AIDS期,可早期诊断. 3.检测病毒的核酸:RealTimePCR-Viral Loading 4. 病毒分离培养:培养液中RT活性 Lab Diagnosis Education Sexually transmitted Drug Abuser Educatio