薯蓣皂甙对大鼠心肌缺血再灌注损伤的保护作用薯蓣皂甙对大鼠心肌缺血再灌注损伤的保护作用.doc

【摘要】 ·kg-1·d-1灌胃，高剂量组薯蓣皂甙300 mg·kg-1·d-1灌胃，低剂量组150 mg·kg-1·d-1灌胃，共7 d。末次给药24 h后，复制大鼠心肌IR损伤模型，观察心脏生理学指标（HR、BP、ST段变化），心律失常评分，心肌梗死面积，心肌形态学指标的变化。结果 与IR组心律失常评分3.75±0.45相比，给药组（DH组2.25±1.18，DL组2.44±1.09）明显下降，心肌梗死面积明显缩小，心功能明显改善。结论 薯蓣皂甙对大鼠心肌IR损伤具有保护作用。 【关键词】 薯蓣皂甙； 心肌缺血； 心肌再灌注损伤 Cardioprotective effects of dioscin against myocardial ischemia reperfusion injury in rats ZHAO Yunqian, KANG Yi, GAO Weizhen, WANG Wei, FAN Xiaojing, LOU Jianshi. Department of Pharmacology, Tianjin Medical University, Tianjin 300070, China Corresponding author: LOU Jianshi, Email: jianshilou@ 【Abstract】 Objective To observe the cardioprotective effects of dioscin on myocardial ischemia reperfusion (IR) injury in rats. Methods 48 Wistar rats were randomly divided into 3 groups: IR group, high dose dioscin (DH) group and low dose dioscin (DL) group. The IR group was treated with NS 10 ml·kg-1·d-1 for 7 days, DH group and DL group were treated with dioscin 300 ml·kg-1·d-1 and 150 ml·kg-1·d-1 respectively for 7 days. The myocardial IR injury rat models were established 24 hours after last dose by the ligation of left descending coronary artery for 30 min and reperfusion for 120 min. Parameters including heart rate, blood pressure, the score of arrhythmia, myocardial infarct size and morphologic change after myocardial IR were observed. Results Compared with IR group （3.75±0.45）， DH （2.25±1.18） and DL groups demonstrated lower score of arrhythmia（2.44±1.09）, a smaller myocardial infarction size and better cardiac function. Conclusion Dioscin can protect myocardium against IR injury. 【Key words】 Dioscin; Myocardial ischemia； Myocardial reperfusion injury 　 近年来，中医药在治疗心肌梗死等缺血性疾病中发挥着越来越重要的作用，其中含薯蓣皂甙(dioscin)的中药作为预防和治疗冠心病的药物已广泛应用于临床和科研［12］。该类药具有活血化淤、行气止痛、扩张冠状动脉血管和改善心肌缺血等功效，可用于预防和治疗冠心病、心绞痛以及淤血内阻之胸痹、眩晕、气短、心悸、胸闷等病症［3］。已有研究表明薯蓣皂甙能够减轻实验性缺氧心肌细胞的钙超载，保护缺氧心肌［4］，同时可使高脂血淤大鼠的高切变率下全血黏度，低切变率下全血黏度，全血还原黏度，血浆黏度明显降低，并能降低其血清总胆固醇和三酰甘油含量［5］。本研究的目的是采用心肌缺血再灌注(ischemia reperfusion，IR)损伤模型