体细胞核移植与中心体遗传.pdf

HEREDITAS (Beijing) 2008年 8月, 30(8): 960―966 ISSN 0253-9772 综 述 收稿日期: 2007?10?30; 修回日期: 2008?05?21 基金项目: 国家自然科学基金(编号:和中国农业科学院北京畜牧兽医研究所“家畜种质资源研究和创新”科技创新团队项目(编号: YZF-TD-1)资助[Supported by the National Natural Science Foundation of China (No. and the Project of Science and Tech- nology Innovation Team “Research and Improvement of Domestic Animal Germplasm” (No. YZF-TD-1), Institute of Animal Science, Chinese Academy of Agricultural Sciences] 作者简介: 杜卫华(1974?), 女, 山西晋中人, 博士, 助理研究员, 研究方向：动物生物技术。Tel: 010 E-mail: dwh679@ 通讯作者: 朱化彬(1965?), 男, 安徽固镇人, 硕士, 研究员, 研究方向：动物生物技术。Tel: 010 E-mail: huabinzhu@ DOI: 10.3724/SP.J.1005.2008.00960 体细胞核移植与中心体遗传 杜卫华, 朱化彬, 郝海生, 王栋 中国农业科学院北京畜牧兽医研究所胚胎工程与繁殖技术研究室, 北京 100094 摘要: 体细胞克隆虽然在多种哺乳动物中成功获得后代, 但仍存在一系列的问题需要解决。克隆胚胎的发育能 力由核移植后几小时内的细胞和分子过程决定, 包括染色体分离和纺锤体的重新组装。中心体的正常组成和分 布能保证染色体分离的准确性及新生和出生后克隆动物发育过程中的基因组稳定性。文章在分析哺乳动物体细 胞克隆存在的问题和简介中心体结构功能的基础上, 综述了中心体在配子和受精卵发育过程中的遗传机制, 同 时阐述了体细胞克隆胚胎中心体及其相关蛋白的研究现状。 关键词: 体细胞核移植; 胚胎发育; 中心体 Somatic cell nuclear transfer and centrosome inheritance DU Wei-Hua, ZHU Hua-Bin, HAO Hai-Sheng, WANG Dong Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China Abstract: The developmental competence of embryos cloned from somatic cells depends on the cellular event and molecu- lar process, such as separation of chromosomes and reorganization of spindle after nuclear transfer. Centrosome, the main microtubule organizing centers in a cell, is crucial for reorganization of spindle and normal separation of chromosomes during mitosis. Aberrant of centrosomes will lead to aneuploidy of blastomere and developmental failure of embryo. This paper expounded the situation of animal somatic cell nuclear transfer (SCNT) and biological functions of centrosome and analyzed the inheritance mechanism of centrosome during gametogenesis and fertilization. Additionally, the study condition of centrosome and its ass