TSK-GEL色谱柱使用中的常见故障及故障排除(中文)100921选编.ppt

TSKGEL色谱柱使用中的常见问题及故障排除;尺寸排阻色谱 (SEC/SW) 离子交换色谱 (IEC) 疏水反应色谱 (HIC) 亲和色谱 (AFC) 反相色谱(RPC) 正相色谱 (NPC/HILIC) 保护柱 FAQ ;HPLC;问题;Conditions Column: TSKgel G3000SWXL (7.8 mm I.D. x 30 cm) Eluent: A; 0.3 mol/L NaCl in 50 mmol/L sodium phosphate buffer, pH 6.7 B; 0.1 mol/L Na2SO4 in 50 mmol/L sodium phosphate buffer, pH 5.0 Flow rate: 1.0 mL/min Temperature: 25 C Detection: UV (280nm) * Allows indicate albumin peaks; ;选择合适的缓冲体系;Column ；TSKgel DEAE-5PW (7.5mmI.D.×7.5cm） Eluent； A:20mmol/l Tris-HCl buffer B:20mmol/l Phosphate buffer A→A+0.5mol/l NaCl（60minlinear gradient） B→B+0.5mol/l NaCl Flow rate；1.0mL/min Temperature；Ambient Detection；pH monitor;BioAssist Q 10x10 Methacrylate type;AIEC;CIEC;问题;Column; TSKgel Phenyl-5PW 7.5 mmI.D. x 7.5 cm Elution; A 60 min linear gradient of ammonium sulfate from 1.5 M to 0 in 0.1 M phosphate buffer (pH 7.0) at a flow rate of 1.0 ml/min, detected by UV absorbance at 280 nm;HIC;色谱柱;问题;问题;Column: TSKgel ODS-100V (4.6mmI.D. x 15cm) Eluent: A; 10mM NaH2PO4/CH3CN=85/15 B; 10mM NaH2PO4/CH3CN=30/70 Gradient: 0min(B ; 0%)→15min(B ; 75%) Flow rate: 1.0mL/min Detector: UV (265nm) Injection vol.: 20μL Concentration: 10μg/L Column temp.: 40℃ 1: sulfisomidine 2: sulfamerazine 3: sulfamethoxazole 4: sulfaquinoxaline ;Column; TSKgel ODS-80Ts (2 mmI.D. x 15 cm) Elution; A 30 min linear gradient of acetonitrile from 0 to 70 % in 0.1 % TFA at a flow rate of 0.2 ml/min, detected by UV absorbance at 215 nm.;Column; TSKgel Phenyl-5PW RP 4.6 mmI.D. x 7.5 cm Elution: A 44 min linear gradient of acetonitrile from 5% to 60 % in 0.02 % TFA at a flow rate of 1.0 ml/min, detected by UV absorbance at 220 nm;问题;更换保护柱;问题;问题;问题