人羊膜上皮细胞移植修复损伤臂丛神经具有转化医学的意义.doc

SICENCE新闻发布 作者：杨琦 稿号：NRR_354_14 人羊膜上皮细胞移植修复损伤臂丛神经：具有转化医学的意义 近年来干细胞移植技术作为中枢和周围神经损伤的治疗手段受到广泛关注，但神经干细胞、胚胎干细胞等存在来源、伦理、免疫排斥和安全性等问题。因此，越来越多的学者开始关注人羊膜细胞。有研究表明，羊膜上皮细胞也具有干细胞的特性，能够分化为成熟的神经细胞，并分泌多巴胺、乙酰胆碱、去甲肾上腺素等神经递质。 来自中国吉林大学中日联谊医院金海博士所领导的团队，将人羊膜上皮细胞悬液注入兔牵拉伤臂丛神经，24周后应用臂丛神经拉伸实验检测到受损神经拉伸强度和弹性明显增强。同时苏木精-伊红染色也证实臂丛神经病理形态明显改善，且兔前爪功能也明显恢复。因此，作者认为人羊膜上皮细胞移植有促进损伤臂丛神经修复和进行转化医学临床应用的潜力。相关文献发表于《中国神经再生研究（英文版）》杂志2014年12月第24期。 以牵拉神经根的方法制备C6臂丛神经损伤动物模型，造模后即刻用微量注射器接无菌玻璃拉丝针头将人羊膜上皮细胞悬液缓慢注入到臂丛神经损伤区，24周后苏木精-伊红染色显示，兔臂丛神经纤维表面模大部分有结缔组织形成的神经内膜包绕髓鞘，形态清晰 Article: Transplantation of human amniotic epithelial cells repairs brachial plexus injury: pathological and biomechanical analyses, by Qi Yang1, Min Luo1, Peng Li2, Hai Jin1 (1 China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China; 2 Department of Mechanics, School of Mechanical Science and Engineering, Jilin University, Changchun, Jilin Province, China) Yang Q, Luo M, Li P, Jin H. Transplantation of human amniotic epithelial cells repairs brachial plexus injury: pathological and biomechanical analyses. Neural Regen Res. 2014;9(24):2159-2163. Transplantation of human amniotic epithelial cells repairs brachial plexus injury Transplantation of stem cells has been of wide interest for the treatment of central and peripheral nerve injuries, but application of neural stem cells and embryonic stem cells is limited by source scarcity, ethical concerns, potential immune rejection, and security. Therefore, more attention has been focused on human amniotic cells as a cell source for transplantation. Several previous studies have shown that amniotic epithelial cells can differentiate into mature nerve cells, secrete neurotransmitters such as dopamine, acetylcholine, and norepinephrine, and have similar biological properties as embryonic stem cells. Dr. Hai Jin, Sino-Japanese Friendship Hospital of Jilin University, China and his colleagues injected a suspension of human amniotic epithe