04-培养基的优化.pdf

ARTICLE Culture Media Optimization for Growth and Phycoerythrin Production from Porphyridium purpureum S. Kathiresan,1 R. Sarada,1 Sila Bhattacharya,2 G.A. Ravishankar1 1Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore, India 570020; telephone: 91-821-2516501; fax: 91-821-2517233; e-mail: sarada_ravi@ 2Grain Science Technology Department, Central Food Technological Research Institute, Mysore, India 570020 Received 27 January 2006; accepted 13 July 2006 Published online 25 September 2006 in Wiley InterScience (). DOI 10.1002/bit.21138 algae), Cryptophyta (cryptomonad algae), and Rhodophyta ABSTRACT: Porphyridium spp. is a red micro alga and is (red algae) (Borowitzka, 1994). The PB consists of gaining importance as a source of valuable products viz., protein moiety and a linear tetrapyrrole prosthetic group phycobiliproteins (PB), sulfated exopolysaccharides, and (phycobilins) attached to the polypeptide through thioether polyunsaturated fatty acids with potential applications in bonds. Based on spectroscopic properties, these proteins are the food and pharmaceutical industries. In the present study, broadly classi?ed into three groups—allophycocyanin the effects of the major media constituents of Porphyridium species were studied using response surface methodology (APC) (650–655 nm), C-phycocyanin (C-PC) (610– (RSM) on biomass yield, total PB and the production of 620 nm), and R-phycoerythrin (R-PE) (540–570 nm). Each phycoerythrin (PE). A second order polynomial can be used of these PB is comprised of two subunits a and b to which to predict the PB and PE production in terms of the linear tetrapyrrole is covalently attached by a cysteine independent variables. The independent variables such as thioether bond. The central portion of all the three groups is the concentrations of sodium chloride, magnesium sulfate, sodium nit