30%毒氟磷可湿粉在烟草和土壤中残留消解分析.pdf

Residue analysis and dissipation of 30% Dufulin wettable 1 powder in Soil and Tobacco Leaves Chen Zhu，Zhang Yanlin，Zeng Song，Song Baoan *，Lu Ping，Hu Deyu， Yang Song，Jin Linhong，Xue Wei Center for Research and Development of Fine Chemicals, Guizhou University，Key Laboratory of Green Pesticide and Bioengineering，Ministry of Education，Guiyang（550025） E-mail：Songbaoan22@ Abstract HPLC-UV method for the analysis of residual Dufulin [O, O’-diethyl-α-(4-methyl benzothiazole-2-ylamino)-(2-fluorophenyl)phosphonate] from soil and tobacco was developed. Residues of Dufulin were extracted from soil (tobacco) matrix with acetone(acetone/petroleum ether), purified by liquid-liquid extraction and Floristl cartridges, and then determined by HPLC with UV detector. The minimum detectable amount of Dufulin was 2×10-10 g, the minimum detectable concentration of Dufulin in the samples of soil (tobacco) were 0.01 mg/kg. The average recovery from the soil and tobacco samples was 75.1~120.8% while coefficient of variation was found to be 1.6~13.4%, meeting requirements for pesticide residue analysis. In the field studies conducted at Guizhou and Shandong Province respectively, when Dufulin dose was applied twice to that recommended for maximum usage (1000 g.a.i/ha 30% Dufulin wettable powder sprayed for once during development of tobacco), the residual Dufulin level collected from the samples after 21d of application was found to be 0.31 mg/kg (0.70 mg/kg) in soil (tobacco). The result showed that the half life of 30% Dufulin wettable powder in soil was 10.0~12.9 days, in tobacco was 4.1~6.0 days. Keywords：Dufulin；tobacco；soil；residue analysis；HPLC；UV. 1. Introduction α-Aminophosphonic acids, bioisosters of natural