HPLC虎耳草四种生物活性物质.pdf

Journal of Pharmaceutical and Biomedical Analysis 50 (2009) 771–777 Contents lists available at ScienceDirect Journal of Pharmaceutical and Biomedical Analysis journal homepage: /locate/jpba Quality evaluation of golden saxifrage (Chrysosplenium alternifolium L.) through simultaneous determination of four bioactive ?avonoids by high-performance liquid chromatography with PDA detection Monika Anna Olszewska ?, Jan Gudej Department of Pharmacognosy, Faculty of Pharmacy, Medical University of ?ód′z, ul. Muszynskiego′ 1, 90-151 ?ód′z, Poland article info abstract Article history: To control the quality of Chrysosplenium alternifolium L., a simple, fast and reliable method of Received 31 March 2009 high-performance liquid chromatography coupled with a photodiode array detector (HPLC-PDA) was Received in revised form 8 June 2009 developed and validated for simultaneous quantitative determination of four bioactive polymethoxy- Accepted 8 June 2009 lated ?avonoids, namely chrysosplenosides B and D, and chrysosplenols B and D. Separation of the four Available online 17 June 2009 analytes was accomplished on a C18 Hypersil ODS column (5 ?m, 125 mm × 4 mm, i.d.) with an acetoni- trile 10–100% (v/v) elution gradient, recorded at 345 nm. The equilibration of the methanol extracts and Keywords: standard solution to 30% (v/v) of water was found to be necessary when minimizing viscosity differences RP-HPLC PDA between