Transwell小室.pdf

Life Sciences Transwell Hands on training Iris Li Application Consultant 09/15/2009 Schematic of Transwell? Inserts Transwell insert Upper (apical) compartment ~1 mm Lower (basolateral) Microporous membrane compartment Life Sciences Corning Incorporated 2 What are permeable supports? ? Permeable supports are thin microporous membranes attached to the bottom of a plastic insert. ? Support types include meshes, track etched membranes as well as stretched membranes. ? Supports come as individual units in multiwell plates or as connected multi-well inserts. ? Corning is the only supplier of THE Transwell permeable support. Long history (20 years) of being the best on the market Life Sciences Corning Incorporated 3 What makes cell growth on a permeable support more in vivo-like? TCT Plastic ? Growth of TCT plastic results in flat morphology and poorly defined junctions ? Epithelial Cells in- vivo are polarized and Top (apical domain) typically columnar ? Serves to form a barrier Tight between the outside and junction Side (basolateral the inside domain) ? Terminal differentiation Bottom (basal domain) ECM Life Sciences Corning Incorporated 4 Chemotaxis studies The1) Thenumber tumor of cells