DNA 纳米技术.ppt

Yongqiang Wen 2015.10.27 ;Challenges;纳米组装体系及其分类;纳米组装体系的重要性;人工组装体系;人工组装 体系;;自组装（自组织）是指通过弱的和较小方向性的非共价键，如范德华力、氢键、?－?相互作用、疏水相互作用和弱的离子键的协同作用把原子、离子或分子连接在一起构成纳米材料或纳米结构。其关键不是大量原子、离子、分子之间弱相互作用力的简单叠加，而是一种整体的、复杂的协同作用。自组装体系形成有两个重要的条件：一是有足够量的非共价键或氢键存在；二是自组装体系的能量较低，否则很难形成稳定的自组装体系;分子自组装组织原理;在适当的基底上，用有机化合物或无机化合物通过自组装技术制备具有有序结构的薄膜;纳米技术——综合交叉学科; A fusion technology of Nanotechnology and Biotechnology. Constructing nanostructured material and utilizing those nanostructured materials in biological, engineering, and medical applications. Theoretical background, innovative uses of DNA in multi-disciplinary filed, recent development ;DNA (脱氧核糖核酸（DNA，为英文 Deoxyribonucleic acid的缩写） 生命遗传物质… 双螺旋结构… DNA 鉴定… DNA 探针… DNA 损伤/修复… DNA 重组… 基因工程… ;;Why DNA? ;;DNA isolation;;;Oligo synthesizers;LCMS characterization of oligos;Chemical Synthesis of Oligonucleotides;;Structural DNA NanotechnologyFunctional DNA Nanotechnology ;;;100 nanometer smiley faces;DNA origami;(Park, Nano Lett. 2005, 5, 729);Turberfield A. J. et al. Science, 2005, 310, 1661;3D DNA Nanostructures;S. M. Douglas, et al. Nature 2009, 459, 414.;TRIANGULAR DNA NANOTUBES;S. Rinker, et al. Nat. Nanotech. 2008, 3, 418. ;Periodic Arrays of Gold Nanoparticles;Discrete Structure of Au Nanoparticles;;;External Control of DNA Organization: WRITE/ERASE;Method to get Au triangle directly ;Significance of the Second Method;a) ;Agarose Gel Electrophoretic Analysis Au-DNA conjugates;? ;Schematic illustration of the construction of AuNP triangle structures and the controlled structural transformation by adding ‘‘fuel’’ and ‘‘antifuel’’ hairpin structures.;DNA Nanomachine ;pH sensitive DNA structurepH sensitive DNA structure;DNA?nanomotor?driven?by?proton;How?fast?it?is? ;Cycle?the?machine;Run?the?motor?at?Solid/liquid?interface JACS 2006,?2067‐2071;DNA?Nanocontainer;Smart?surface?based?on?DNA?motor?Angew.?Chem.,?Int.?Ed.?46,?3915‐3917;Smart surface based on DNA motor ; DNA aptam