云龙黑木耳中多糖的提取与测定.PDF

大理学院学报 第11卷 第3期 2012年3月 JOURNAL OF DALI UNIVERSITY Vol.11 No.3 Mar. 2012 云龙黑木耳中多糖的提取与测定 1 1，2* 2* 1，2 1 ， 倪兆林 ，申元英 ，白 丽 ，陈红云 李润兵 （1.大理学院公共卫生学院，云南大理 671000 ；2.大理学院基础医学院，云南大理 671000） ［摘要］目的：提取云龙野生和人工培养两种黑木耳多糖并测定其含量。方法：采用乙醇浸提法提取多糖，紫外分光光度苯酚-硫 酸显色法测定粗多糖含量。结果：在8.0~48.0 μg/mL线性范围内，回收率为99.03% ，RSD为0.66% （n=6），测到野生和人工培养黑 木耳中多糖的收率分别为18.48%和18.29%；野生和人工培养黑木耳中多糖的含量分别为9.21%和8.20%。结论：野生黑木耳比人 工培养黑木耳中多糖的含量高，生长环境对黑木耳多糖含量有影响。 ［关键词］黑木耳；乙醇浸提法；紫外分光光度苯酚-硫酸显色法；多糖；含量 ［中图分类号］R284.2 ［文献标志码］A ［文章编号］1672-2345 （2012）03-0031-04 Extraction and Determination of Polysaccharides from Yunlong Auricularia auricular 1 1，2* 2* 1，2 1 NI Zhaolin , SHEN Yuanying ，BAI Li , CHEN Hongyun ，LI Runbing （1. Collegle of Public Health, Dali University, Dali, Yunnan 671000, China; 2. Pre-clinical Collegle, Dali University, Dali, Yunnan 671000, China） 〔Abstract 〕Objective: To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their contents. Methods: The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol -sulfuric acid color method. Results: Within the range of 8.0 -48.0 μg/mL, the recovery ratio of polysaccharide was 99.03% with RDS of 0.66% （n=6）. The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%, respectively. Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% an