柱前衍生化–高效液相色谱法测定蒙古黄芪多糖中单糖的组成.PDF

? 468 ? 现代药物与临床 Drugs Clinic 第 27 卷 第 5 期 2012 年 9 月 柱前衍生化–高效液相色谱法测定蒙古黄芪多糖中单糖的组成 陈虎虎 1,2，孙 静 3，杨金颖 1，龚苏晓 2，张铁军 2* 1. 江苏豪森药业股份有限公司，江苏 连云港 222001 2. 天津药物研究院，天津 300193 3. 天津医科大学，天津 300070 摘 要：目的 测定蒙古黄芪多糖中的单糖组成。方法 蒙古黄芪多糖样品用三氟乙酸溶液水解成单糖，用 1-苯基-3-甲基- 5-吡唑啉酮（PMP）衍生化，采用 HPLC 法于 245 nm 波长处检测各单糖。结果 蒙古黄芪多糖由甘露糖、鼠李糖、半乳糖 醛酸、葡萄糖、阿拉伯糖组成，物质的量比分别为 0.02∶0.05∶0.17∶1∶0.18。结论 此方法简单、快速、重复性好，可用 于蒙古黄芪多糖中单糖组成分析。 关键词：蒙古黄芪多糖；柱前衍生化；单糖组成；高效液相色谱 中图分类号：R286.02 文献标志码：A 文章编号：1674 - 5515(2012)05 - 0468 - 03 Determination of monosaccharide compositions in Astragalus mongholicus var. mongholicus polysaccharides by pre-column derivatization-HPLC CHEN Hu-hu1,2, SUN Jing3, YANG Jin-ying1, GONG Su-xiao2, ZHANG Tie-jun2 1. Jiangsu Hansoh Pharmaceutical Co., Ltd., Lianyungang 222001, China 2. Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 3. Tianjin Medical University, Tianjin 300070, China Abstract Objective To determine the monosaccharide compositions in Astragalus mongholicus var. mongholicus polysaccharides (AMMP). Methods AMMP samples were first hydrolyzed with trifluoroacetic acid, and then derivatized with 1-phenyl-3-methyl-5- pyrazolone (PMP). Monosaccharide was determined by HPLC at detective wavelength of 245 nm. Results AMMP composed of mannose, rhamonse, galacturonic acid, glucose, and arabinose with molar ratio 0.02∶∶∶∶ 0.05 0.17 1 0.18. Conclusion The method is simple, fast, and reproducible, and could be used for the analysis of monosaccharide compositions in AMMP. Key words: Astragalus mongholicus var. mongholicus polysaccharide s (AMMP); pre-column derivatization; monosaccharide compositions; HPLC 蒙古黄芪 Astrgalus membranaceus (Fisch.) Bge. 成分[1-3]。多糖是蒙古黄芪的主要生物活性成分之 var. mongholicus (Bge.) Hsiao 是膜荚黄芪的