3种方法提取辣椒根总RNA的效果比较.pdfVIP

…试验棋克、 2016 ,29( 1): 8-10 中国瓜菜 3 种方法提取辣椒根总 RNA 的效果比较 孙国胜，马志虎，戴忠良，毛忠良，孙春青，潘跃平 (江苏丘陵地区镇江农业科学研究所 江苏句容 2124C则) 摘要:针对辣椒根中富含多糖多盼的特点，比较了 Trizol 法、改良 Trizol 法和改良 CTAB 法 3 种方法对辣椒根 总 RNA 提取的效果。结果表明， Trizol 法、改良 Trizol 法能提取到辣椒根总 RNA ，但效果不理想;改良 CTAB 法提取 到的辣椒根总 RNA ， 28SrRNA 的亮度是 18 SrRNA 的 2 倍， OD20010D 28O和 OD20010D230 值分别为 1. 88 和1. 80 ， RNA 质 量浓度为 254.0 mgL-l; 用提取到的 RNA 进行 RT-PCR 后可扩增出 acting 基因特异片段。因此改良 CTAB 法提取 辣椒根总 RNA 的效果好、质量高、完整性好，能够满足后续试验要求。 关键词:辣椒根;改良 CTAB 法 ;RNA 提取;比较 Comparison of three methods 08 total RNA extraction from the root of Capsicum annuum L. SUN Guosheng, MA Zhihu , DAI Zhongliang, MAO Zhongliang, SUN Chunqing, PAN Yueping (Zhenjiang li阳tiu出 0/Agricultural Sciences in Hüly Area 0/ Jia，啄.su Provi配e ， Jurong2124ω ， Jia，啄拙 ， China) Abs回ct: As the characteristic of rich polyphenol and polysaccharide in the tissue of root in Capsicum annuum L., three meth? ods including Trizol ,optimized Trizol and optimized CTAB to extract total RNA were compared. The results showed that the to? tal RNA with high yields and better quality was extracted by optimized CTAB method that 由 e 28 S rRNA band showed two folds than that of 18S rRNA 四 e ratio of ODz.JOD 28O and ODz.JOD230 盯e 1.88 and 1.80, the output rate of RNA was 254.0 问- 1 mL- ? Specific acting gene fragment was 缸npl凶ed by reverse transcription PCR. Above all , the RNA extracted by optimized CTAB method is intact with high quality and output rate. It can be used in following molecular biology experiments. Key words: Capsicum αnnuum L.; Optimized CTAB method; RNA extraction; Comparison 高质