hippocampalneuronsoffetalrat.PDF

Zhang et al. / J Zhejiang Univ-Sci B (Biomed Biotechnol) in press 1 Journal of Zhejiang University-SCIENCE B (Biomedicine Biotechnology) ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) /jzus; E-mail: jzus@ Neuroprotection of dexmedetomidine against propofol-induced neuroapoptosis partly mediated by PI3K/Akt pathway in hippocampal neurons of fetal rat* Ning ZHANG§1,2, Quan-ping SU§3, Wei-xia ZHANG4, Nian-jun SHI2, Hao ZHANG2, Ling-ping WANG2, Zhong-kai LIU2, Ke-zhong LI??5 (1The Second Hospital of Shandong University, Jinan 250033, China) (2Department of Anesthesiology, Linyi People’s Hospital, Linyi 276003, China) (3The central Laboratory, Linyi Peoples’ Hospital, Linyi 276003, China) (4Intensive Care Unit, Linyi People’s Hospital, Linyi 276003, China) (5Department of Anesthesiology, the Second Hospital of Shandong University, Jinan 250033, China) ?E-mail: likezhongsdu@163.com Received Oct. 19, 2016; Revision accepted Dec. 18, 2016; Crosschecked Jan. 6, 2017 Abstract: The aim was to investigate how the PI3K/Akt pathway is involved in the protection of dexemedetomidine against propofol. The hippocampal neurons from fetal rats were separated and cultured in a neurobasal medium. Cell viability was assayed by MTT. Then neurons were pretreated with different concentrations of dexem