RNA基础知识-WorkingwithRNAthebasics.PDF

TECHNICAL NOTE ® Ambion products and technologies Working with RNA: the basics Avoiding, detecting, and inhibiting RNase RNases, which play important roles in nucleic acid • Wear gloves during experiments to prevent metabolism, are found in both prokaryotes and contamination from RNases found on human hands eukaryotes, and in practically every cell type. The • Change gloves after touching skin (e.g., your face), human body uses RNases to defend against invading doorknobs, and common surfaces microorganisms by secreting these enzymes in • Have a dedicated set of pipettes that are used solely for RNA work fluids such as tears, saliva, mucus, and perspiration. • Use tips and tubes that are tested and guaranteed to be RNases are also present in flaked skin, on hair that RNase-free may fall onto a bench, and in pet hair that may cling • Use RNase-free chemicals and reagents to clothing. The primary source of RNases within • Designate as an “RNase-free zone” a low-traffic area most environments, however, is microorganisms— of the lab that is away or shielded from air vents or namely, bacteria and fungi. In this article, we discuss open windows how to avoid, detect, and inhibit RNase when working with RNA. Avoiding s