不同来源的金的黄色葡萄球菌耐药率比较分析.pdf

#论 著# 不同来源的金黄色葡萄球菌耐药率比较分析 陈 坚1 , 王彩虹2 , 王金果1 , 余方友1 ( 1. 温州医学院附属第一医院实验诊断中心, 浙江 温州 325000; 2. 温州医学院生命科学学院、 检验医学院, 浙江 温州 325000) 摘要: 目的 探讨不同标本来源的金黄色葡萄球菌耐药谱。方法 采用常规方法分离金黄色葡萄球菌, 用全自 动微生物分析仪进行菌种鉴定和药敏试验, 采用苯唑西林纸片扩散法检测耐甲氧西林金黄色葡萄球菌( MRSA ) , 统计分析用 V 2 检验。结果 分离金黄色葡萄球菌 248 株, 标本随机挑取自血液、导管、脓液、痰液、尿液, 分别为 25、27、89、93、14 株;所有分离株对呋喃妥因和万古霉素敏感, 脓液分离的金黄色葡萄球菌对氯霉素、庆大霉素、 苯唑西林、青霉素、利福平、四环素、左氧氟沙星的耐药率显著低于痰液分离株的耐药率( P 0. 05) ; MRSA 的总 分离率为 68. 0% , 脓液分离的 MRSA 对氯霉素、庆大霉素、青霉素、利福平、四环素、左氧氟沙星的耐药率显著低 于痰液分离株的耐药率( P 0. 05)。结论 脓液分离的金黄色葡萄球菌和 M RSA 耐药率普遍低于痰液分离株的 耐药率。 关键词: 金黄色葡萄球菌; 耐甲氧西林金黄色葡萄球菌 ; 纸片扩散法; 耐药率 中图分类号: R978. 1 文献标识码: A 文章编号: 1005-4529( 2009) 05-0571-03 Antimicrobia-l resistant Profile of Staphy lococcus aureus from Different Origins CHEN Jian* , WANG Ca-i hong, WANG Jin-guo, YU Fang-you (* T he Fir st A f f il iated H osp i tal , Wenz hou M edical Col lege, Wenz hou , Zhej iang 325000, China) Abstract: OBJECTIVE To investigate the antimicr obia-l resistance of S taphy lococcus aur eus isolat es which obtained fr om differ ent o rig ins. METHODS The isolates o f S. aur eus w ere der iv ed fr om clinical samples by routine methods. The identif ication and susceptibility test of the iso lates wer e determined by Automated M icr obiolog y Ana lyzer. Disk-diffusion t est ( K-B method) w as used for detecting met icillin- r esistant S. aur eus ( MRSA ) . V2 Test w as made to identify the significance o f difference. RESULTS A tot al o f 248 S. aur eus str ains were isolated fr om blood ( 25) , medical internal catheter ( 27) , pus ( 89) , sputum ( 93) , and urine ( 14) . A ll isolates o f S. au- r eus w ere sensit ive to nitr ofurantoin and vancomycin. The r esistant r ates of S . aur eus isolat es fr om pus to chlo ramphenicol, g ent amicin, ox acillin, penicillin, rifampicin, tetracycline and levo flo xacin all significantly low er than tho se o f isolates fr om sputum ( P 0. 05) . 68. 0% Of iso lates wer e r eferr ed as MRSA. T he r esistant rates of MRSA fr om pus to chlor amphenicol,