安徽省部分地区致病性大肠杆菌耐药性分析.PDFVIP

安徽农业大学学报, 2013, 40(3): 430-433 Journal of Anhui Agricultural University 网络出版时间：2013-4-26 15:37:18 [URL] /kcms/detail/34.1162.S1537.021.html 安徽省部分地区致病性大肠杆菌耐药性分析 * 阮祥春，曾明华 ，黄媛媛，孟艳静，李 琳，李 郁 (安徽农业大学动物科技学院，合肥 230036) 摘 要：为了研究临床大肠杆菌对药物的敏感性，从安徽省部分地区病料中分离得到 75 株致病性大肠杆菌， 7 CFU·mL-1 的菌液，采用 K-B 法开展了对 13 种药物的敏感性试验。结果显示， 经分离、纯化、增殖，制成 5 ×10 分离株对氨苄西林、阿莫西林、多粘菌素 B 耐药菌高达 70%以上，对恩诺沙星、氟苯尼考、氧氟沙星、头孢曲松、 头孢噻肟的耐药菌株在 30%以上，而对阿米卡星、头孢唑肟高度敏感。本试验为临床大肠杆菌病防治有一定意义。 关键词：大肠杆菌；药敏；耐药；抑菌圈 中图分类号：S852.61 文献标识码：A 文章编号：1672−352X (2013)03−0430−04 Drug sensitivity of Esherichia coli in some districts of Anhui Province RUAN Xiang-chun, ZENG Ming-hua, HUANG Yuan-yuan, MENG Yan-jing, LI Lin, LI Yu (School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036) Abstract: In order to investigate drug sensitivity of pathogenic Escherichia coli from animal clinics,75 pathogenic Escherichia coli isolates were obtained from 117 samples from 15 districts of Anhui Province. The 7 -1 bacteria liquid was prepared in the concentration of 5×10 CFU·mL after isolation, purification and proliferation, to analyze its sensitive to 13 drugs by K-B method. The inhibition zones were determined after overnight cultiva- tion. The results showed that the rates of drug resistant strains were above 70% to ampicillin, amoxicillin and po- lymyxin B, above 30% to enrofloxacin, florfenicol, ofloxacin, ceftriaxone and cefotaxime, and sensitive to ami- kacin and ceftizoxime. The investigation demonstrated that it is necessary for rational use of antimicrobial agents for the control of colibacillosis in pigs and chickens. Key words: Escherichia coli ; drug