扑草净降解菌降解特性_降解基因保守序列的扩增及同源性分析_颜慧.pdf

15 4 V l 15, N .4 2002 8 URBAN ENVIRONMENT URBAN ECOLOGY Apr.　2002 61 、 1 1 1 2 1 颜　慧, 李军红, 宋文华, 温孚江, 朱　坦 (1.南开大学环境科学与工程学院, 天津　300071; 2.山东农业大学,泰安　271018) 　　:FR, , pH , pH 7.0, 32℃;, PCR 500 bp , , atzA、triA 。 :;;; :X17　　:A　　:1002-1264(2002)04-0061-03 Degrading Characters of a Degrading-Prometryn Strain and Amplification Homology Analysis of the Degrading-Gene Conservative Sequence 1 1 1 2 1 YAN Hui , LI Jun-h ng ,SONG Wen-hua , WEN Fu-jiang , ZHU Tan (1.C llege f Envir nmental Science and Engineering, Nankai University, Tianjin 300071,China; 2.Shand ng Agricultural University, Taian 271018,China) Abstract:A degrading-pr metryn strain, is lated fr m s ils exp sed t repeated spills f the herbicide, bel ngs t Pseud m nas.It was pr ved that the degrading effect was affected by temperature and pH, and that the ptimum c n- diti n was pH 7.0, 32 ℃.After designing the primers, a 500 bp-length degrading-gene c nservative sequence was btained by PCR amplificati n.With partial sequencing and h m l gy analysis, the result sh wed there is a high h - m l gy between this sequence and the genes f s-triazine hydr lases such as atzA, triA. Key words:pr metryn;　degrading strain;　c nservative sequence;　h m l gy 　　, DNA 　Sang n 12 18 。 。 , Silver Beads DNA 　Sang n , 。 [1] , (1 L ): 。 (Na2HPO4 1 g,