晚期大肠癌ERCC5基因启动子__省略_多态性与铂类药物疗效相关性的研究_陈建芳.pdf

DO I :10.16073/j .cnki .cj cpt.2010.24.005 ·20 10 · , 　ERCC5 -763AG 〖实验研究〗 ERCC5 -763AG 陈建芳, 　李建军, 　蒋金妍, 　邹岚, 　裴莉, 　潘凤, 　梁后杰 第三军医大学西南医院肿瘤中心, 重庆400038 Relationship between ERCC5-763AG polymorphism and clinical outcome of patients withadvanced colorectal cancer treated with platinum-based chemotherapy CH EN J ian-f ang, LI J ian-j un, J IA N GJ in-y an, ZOU Lan, PE I L i, P AN Feng, LI AN G Hou-j ie Cancer Center, Southwest Hos ital, Third Military Medical University , Chongqing 400038, P.R.China 【】　:探讨ERCC5 基因启动子 [ABSTRACT] 　OBJECTIVE:To study the r e atio nship betw een sing e 区单核苷酸多态 与中国汉族晚期大肠 nuc eo tide po y mor phism s (SN P)in the pr omo te r of ERCC5 (excision 癌奥沙利铂疗效的相关 。 :收集 repair cr oss comp ementatio n g roup 5 )g ene and c inica o utco me o f 以奥沙利铂为主化疗的105 例晚期大肠 Chinese patients w ith advanced co orecta cancer treated w ith o xa ip a- 癌患者化疗开始前静脉血, 提取基因组 tin-ba sed chemo ther apy .METHODS:DN A w as e xtr acted fro m pe- D NA, 应用P CR-LD R 方法检测ERCC5 riphera b oo d ce s befo re ox a ip atin-based chemotherapy in 105 ad- 基 因 3 个 SNP 位 点-14 15C T vanced co o recta cancer patients.T hree po ymo rphism s in the ERCC5 (r s209425 8)、-763A G (rs2016073)及 gene promo ter, -14 15CT (r s2094258), -763AG (r s2016073)and -4 13CT (rs94324 5)多态 ,分析不同 -413CT (r s94324 5)w ere detected by PCR-LDR (po y me rase chain 基因型与疾病控制率、无进展生存期 reactio n-igation detection reactio n).A sta tistica ana y sis w as conduc- (PFS )的相关 。 :携带 ERCC5 ted to inv estiga te the asso ciatio n betw een po ymo rphism and c inica -763GG 、-763AG 和-763A