植物甾醇与三萜类皂苷生物合成基因调控的研究进展_刘强.pdf

, Journal of Anhui Agri.Sci.2006,34(19):4844-4846　　　　　　　　　　　　　　　　　　　孙红忠　　孙红忠 ＊ 刘强, 丛丽娜 ,张宗申　(, 116034) 　植物甾醇和三萜类皂苷是2 种具有调节生物体系免疫力, 抗 糖过多, 抗癌症等生理功能的植物次生代谢产物。 它们在生物合 成过程中有许多关键酶, 如鲨烯合成酶(SS)、鲨烯氧化酶(SE)、氧化鲨烯环化酶(OSCs)和糖基转移酶(GT)等。综述了这些关键酶在催化 机理、基因克隆与表达调控方面的研究进展, 并简要探讨了通过这些关键酶的代谢工程研究来增产植物甾醇和三萜类皂苷的广阔前景。 　植物甾醇;三萜类皂苷;鲨烯合成酶;鲨烯氧化酶;氧化鲨烯环化酶;糖基转移酶 　Q786　　　A　　　0517-6611(2006)19-4844-03 Research Progress in Genetic Analysis of the Biosynthesis of Phytosterols and Triterpene Saponins LIU Qiang et al 　(College of Bio Food Technolog , Dalian Institute of Light Industr , Dalian, Liaoning 116034) Abstract　The ph tosterols and triterpene saponins are two kinds of plant secondar metabolites, which have the immune s stem modulation functions, anti-h pergl cemic activities and anti-cancer effects in the organism.There are several pivotal enz mes in the bios nthesis, such as squalene s nthase (SS), squalene epoxidase (SE), oxidosqualene c clases (OSCs), glucos ltransferase (GT)and so on.Here, we summarized the research progress in the catal sis mechanism, gene cloning, expression and regulation of those enz mes.In addition, in this paper also the extensive background of increasing pro- duction of the ph tosterols and triterpene saponins through the engineering metabolites of the important enz mes were briefl discussed. Key words　Ph tosterols;Triterpene saponins;Squalene s nthase;Squalene epoxidase;Oxidosqualene c clase;Glucos ltransferase 　　, , , , , , 。 [1] 。 ,, 。 。 1 　 (plant primar metabolism) (plant secondar metabolism)。 , 、、、。 , , 。 , [2] 。 , (Mevalonic acid) (IPP)(GPS ) (GPP), (FPS ) 1　 (FPP),(Squalene s nthase,SS ) 　 ,(Squalene epoxidase,SE) ,, [3] 2,3-(2,3-oxidosqualene) 。SS SE (SS )(SE) ,