植物石蜡切片的固定与保存_王原媛.pdf

198 , AnhuiAgri1Sci1Bull12010, 16( 1) * 王原媛 张定宇 黄春国 (, 030801) : 石蜡切片是观察植物组织构造常用的制片方法之一活的细胞或组织多为无色透明, 种组织间和细胞内 种结构之间均缺乏反差, 在 一般光镜下不易清楚区别; 组织离开机体后很快就会死亡和产生组织腐败,失去原有正 常结构, 因此, 组织要经固定石蜡包埋切片及染色等步骤以免细胞组织死亡, 才能清晰辨认其形态结构重点研究 了植物石蜡切片的固定与保存,拟为今后固定剂及固定方法的选择提供科学依据 : 石蜡切片; 固定; 保存 Q94311 A 1007- 7731( 2010)01- 198- 03 Research of PlantParaffin Section s Fixation and Preservation W ang yuanyuan et al. ( Shanx iAgricultural niversity, Taigu 030801, China) Abstract: paraffin section is one of themethods commonly used in observation ofplant tissue. L iving cells or tissues aremost- ly colorless, transparent, and cells among the various organizationsw ithin the lack of contrast bewt een the various structures, nder normal lightm icroscope, not easy to clearly distinguish The organization willdie soon after leaving the body and pro- duce the organization of corruption, loss of normal structure of the original, therefore, the organization must be subject to fixed, embedded in paraffin, sliced and stained tissue death and other steps to avoid, but can clearly identify theirmorpho- logical structure. This paper focuses on fixed paraffin sections ofplant and preservation for future fixed- agents and a fixed method of providing a scientific basis. Key words: Paraffin sections; fixed; save ( paraffin section) , , , , ( , 015Lm), , , , , , , , , , 2 ,, , , , , , , , ,