1 483-485 z512 基础研究 3+18 胡萝卜素降解物诱导小鼠肝癌细胞H22产生免疫活性的研究.pdf

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.5 胡萝卜素降解物诱导小鼠肝癌细 胞H 产生免疫活性的研究 22 刘安军，张倩云，郑国强，董慧，方菁菁，滕安国，郑捷，王稳航 （天津科技大学食品工程与生物技术学院，天津300457） 摘要：本文研究了以胡萝卜素降解物作为免疫诱导剂的H22 全细胞免疫物对小鼠H22 肝癌的抑制作用，通过用胡萝卜素降解物 与H22 细胞共培养后对小鼠进行免疫，同时设立模型组和空白组，试验期间，测定小鼠生长曲线、脏器指数、抑瘤率、巨噬细胞吞噬 能力、NK 细胞杀伤活性、免疫器官HE 染色等。结果表明：胡萝卜素降解物对小鼠没有毒性作用，免疫组小鼠抑瘤率为93%、巨噬 细胞吞噬指数和廓清指数分别为5.42±0.19、0.39±0.002，NK 细胞杀伤活性为0.93，总体各个指标均与模型组小鼠呈显著性差异，说 明H22 全细胞免疫物能够增强小鼠的免疫能力，有效保护免疫器官。 关键词：胡萝卜素降解物；免疫诱导剂；免疫能力 文章篇号：1673-9078(2012)5-483-485 Immune-activation Effects of Carotene Degradants on H22 Tumor Cells LIU An-jun , ZHANG Qian-yun, ZHENG Guo-qiang, DONG Hui FANG Jing-jing, TENG An-guo, ZHEN Jie, WANG Wen-hang (College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China) Abstract: The effects of H22 cells vaccine treated with carotene degradants as immunoadjuvant on the mice were studied in this paper. By immunising the mice with the coculture of H22 cells and carotene degradants, setting the model and the control, it was found that the carotene degradants had no toxic effects on the mice and the cancer inhibition rate of immue mice was 93%. Carbon particle clearance and the phagocytsis index of macrophages were 0.39±0.002 and 5.42±0.19, respectively . Cytotoxicity of NK cells was 0.93. All of the parameters had significant difference compared with those of the model. The research proved that H22 cells vaccine treated with carotene degradants can effectively protect immune organs. Key words: carotene degradants; immunoadjuvant; immunopotency 类胡萝卜素广泛分布于大自然中，其主要功能是 萝 卜素降解物的研究开辟了新思路。 对单线态氧的有效淬灭，能消除羟自由基，是脂类过