5_7_二羟基_4_甲氧基二氢黄酮与牛血清白蛋白的相互作用研究.pdf

2006 64 Vol. 64, 2006 13 , ACTA CHIMICA SINICA No. 13, · · 5,7--4- a a a,b a *,a,b (a 475001) (b 475001) 5,7--4- (ISO)(BSA) . : ISO BSA ISO BSA ; 7.41 1011 L/mol, 1.98. ISO BSA 7-OH 5-OH, 7-OH 5-OH, ISO , BSA . 5,7--4- ; ; Studies on the Interaction between 5,7-Dihydroxy-4-methoxy- flavanone and Bovine Serum Albumin WANG, Yonga LI, Lin-Xia ZHAO, Dong-Baoa,b ZHANG, Weia LIU, Xiu-Hua*,a,b (a College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475001) (b Institute of Natural Products and Medicine Chemistry, Henan University, Kaifeng 475001) Abstract The interaction between 5,7-dihydroxy-4-methoxyflavanone (isosakuranetin, ISO) and bovine serum albumin isosakuranetin (BSA) has been investigated by fluorescence, UV absorption spectra and NMR spectroscopy. The results show that the mechanism of quenching the inner fluorescence of BSA is due to the combination of BSA with ISO which results in static quenching procedure. The binding constant is 11 7.41 10 L/mol and the number of binding sites is 1.98. The active groups of ISO interacting with BSA are 7-OH and 5-OH, and 7-OH is more active than 5-OH. With the increase of ISO concentration, the conforma- tion of BSA has been changed. Keywords 5,7-dihydroxy-4-methoxyflavanone; bovine serum albumin; interaction , , , , , . [5] [6] [7] [8] [9] HIV ,