5种海洋微藻多糖体外免疫调节活性的筛选_周妍.pdf

第29 卷 第2 期 海 洋 通 报 Vol. 29, No. 2 2010 年 04 月 MARINE SCIENCE BULLETIN Apr. 2010 5 种海洋微藻多糖体外免疫调节活性的筛选 周妍，王凌，孙利芹，王长海 （烟台大学海洋学院，山东烟台 264005 ） 摘 要： 目的：研究5 种海洋微藻多糖对小鼠体外免疫细胞功能的影响，筛选出免疫调节活性强的微藻藻株。方法：考察 5 种海洋 微藻多糖对小鼠脾脏淋巴细胞增殖，腹腔巨噬细胞 RAW264.7 增殖、吞噬中性红、释放 NO 能力的影响。结果：5 种海洋微藻多糖对 小鼠免疫细胞具有不同的刺激能力，其中，紫球藻多糖可极显著增强吞噬细胞的吞噬功能，促进巨噬细胞合成 NO ，促进脾淋巴细胞 及腹腔巨噬细胞的增殖。证明紫球藻多糖具有增强小鼠细胞免疫功能的作用。 关键词：微藻多糖；脾淋巴细胞；腹腔巨噬细胞；免疫调节；活性； 中国分类号：Q949.2 文献标识码：A 文章编号： 1001-6932(2010)02-0194-05 Immunomodulation activities of polysaccharides in vitro from five micro-algae ZHOU Yan, WANG Ling, SUN Li-qin, WANG Chang-hai (Marine School of Yantai University, Yantai 264005, China) Abstract: To study the effects of polysaccharides from five micro-algae on immunocell functions of mice in vitro, and screen out bioactive marine micro-alga. The immune stimulating activities of polysaccharides from five micro-algae toward mice cells ，including stimulating spleen lymphocyte proliferation and macrophage proliferation, enhancing phagocytic activity of neutral red, increasing nitro oxide (NO) production were tested. The results showed that the polysaccharides from these five micro-algae had stimulating activities on cells mediated immunity in mice. The Porphyridium creuntum polysaccharides had the effect of enhancing phagocytic activity of neutral red, increasing nitro oxide (NO) production, and stimulating spleen lymphocyte proliferation and macrophage proliferation. It proved that Porphyridium creuntum polysacchari