MTT法在抗病毒药物筛选中的应用_刘钊.pdf

DOI :10.14188/j.1671 -8852.2004.03.035 25 3 () Vol.25 No.3 　2004 5 Medical Journal of Wu an University May, 2004 MTT 法在抗病毒药物筛选中的应用 1 1 1 1 2 3 刘　钊 　杨占秋 　肖　红 　文　莉 　李汝霖 　姜　旋 1 2 ;　　430071 3　　430072 　目的:M TT (CVB )、(RSV )。 3 方法:, , , (CPE) , M TT , OD570, 。 结果:M TT CPE , OD , , 。 结论:MTT 。 　MTT ;;; 　Q 784 　　A　　1671-8852(2004)03-0332-04 A Simple and Rapid Method of MTT Assay for Evaluation of Antiviral Activity Liu Zhao, Yang Zhanqiu, Xiao Hong, et al I nsti tu te of Virology , S chool of M ed icine, Wuhan Un iversity , Wuhan 430071, Ch ina A stract O jective:To establis ed a rapid and sensitive met od for evaluation of antiviral activity.Meth- ods:T e antiviral activity can be assesed as follow ing:After t e Hep-2 cells (at a dentisty of 2×105 seeded in 40-well tissue culture plates)were infected w it CVB and RSV and incubated for 1.5 at 3 37 ℃.T e virus w ere removed and different concentration of t e test samples w ere added and incu- batded again.W en t e cytopat ic effect (CPE)could be observed at almost every test wells, 50 μl of t e MT T solution (5 mg ·ml-1)was added to eac well.After 3 ours incubation, t e MTT solution w as removed and 100 μl DMSO w as added to eac well.10 minutes later, t e absorbance of t e w ells w as recorded at 570 nm w avelengt s for calculating t e in ibitory rate of t e virus.Results:T e MTT assay w as more simple, rapid, ef