5个X-STR基因座荧光复合扩增体系的建立及法医学应用.pdf

HEREDITAS (Beijing) 2007 12 29(12): 1459 ―1462 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1459 5 X-STR 刘秋玲, 吕德坚, 孙宏钰, 陆惠玲, 伍祥林, 伍新尧 , 510089 为了发掘更多多态性高的 X 染色体短串联重复(X-STR)基因座, 以解决法医学实践中的特殊案例, 建立 了一组荧光复合扩增体系, 同时检测DXS6803 、DXS981 、DXS6809 、DXS6789 和DXS7132 5 个X-STR 基因座, 并用ABI PRISM 3100 作毛细管电泳和GeneMapper ID 3.1 软件进行基因分型, 结果清晰, 灵敏度高, 重复性好。 最低检出限为0.25 ng, 10~20 ng 模板DNA 能得到最佳结果, 在实际检案中能得到满意结果。实验表明, 本体系 能为用X-STR 基因座解决特殊的亲权鉴定案提供快速鉴定技术, 是常染色体 STR 、Y-STR 等鉴定方法的良好 补充, 在法医学实践中有较好的实用价值。 : X 染色体短串联重复(X-STR)；荧光复合扩增；亲权鉴定；法医学应用 Development and forensic application of a pentaplex X-STR loci typing system LIU Qiu-Ling, LV De-Jian, SUN Hong-Yu, LU Hui-Ling, WU Xiang-Lin, WU Xin-Yao Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China Abstract: A fluorescent multiplex PCR that simultaneously amplifies five X-chromosomal short tandem repeat (X-STR) loci (DXS6803, DXS981, DXS6809, DXS6789 and DXS7132)was set up to study their polymorphic nature and to determine its use in kinship tests for forensic cases. PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer with GeneMapper ID 3.1 Analysis Software. The pentaplex system gave satisfactory results as its sensi- tivity, reproducibility and unambiguous genotyping. About 20 ng DNA was routinely used, although 0.25 ng DNA was suf- ficient for allele typing. The results demonstrate that the multiplex system of the five X-STR loci provides a fast technology for forensic identification and paternity testing. The X-STR pentaplex system can complement the analysis of AS-STR and Y-STR efficiently, especially in complex cases of kinship testing. Keywords: X-STR; fluorescent multiplex PCR; kinship testing; fore