白蚁肠道可培养菌群及其功能初探_刘冰.pdf

生 物 灾 害 科 学 2012, 35(2): 172 －176 Biological Disaster Science, Vol. 35, No. 2, 2012 swzhkx@163.com DOI ：10.3969/j.issn.2095-3704.2012.02.011 白蚁肠道可培养菌群及其功能初探 刘冰，林景栋，宋水林，吴德龙 （江西农业大学农学院，江西 南昌 330045 ） 摘要：白蚁是一种危害严重的昆虫，其以破坏木质而家喻户晓。文献表明，白蚁对木质强大的消化能力很大程度 上取决于其肠道微生物的影响。为了探究白蚁肠道微生物的类群，本试验以黑翅土白蚁为材料，分离纯化获得其 肠道主要可培养微生物的纯培养 112 种，其中喜氧菌 35 种，厌氧菌 77 种；在分离出的微生物类群中，通过刚果 红法初步筛选出 12 种喜氧菌菌株可能具有纤维素酶活性，其最大透明圈达到 4.0 ㎝×4.0 ㎝；进一步检测这些菌株 的纤维素酶活性发现，12 株菌均具有羧甲基纤维素钠盐（CMC-NA ）酶活性和滤纸酶活性，但是大部分菌株羧甲 基纤维素酶活性较弱，而一些菌株如 NBY14 滤纸酶活较高，可达到 16.188u/mL。试验结果可为黑翅土白蚁防治 提供依据，亦可为纤维素酶的开发利用奠定基础。 关键词：白蚁；肠道菌群；刚果红法；羧甲基纤维素酶；滤纸酶 中图分类号：S476.1 文献标志码：A 文章编号：2095 －3704 （2012 ）02 －0172 －05 The Microbes Cultured and Their Function in the Gut of Termites LIU Bing, LIN Jing-dong, SONG SHui-lin, WU De-long (College of Agriculture，Jiangxi Agriculture University, Nanchang 330045, China) Abstract: Termites, an important pest, were known by everyone because of their damage to the wood. It is suggested that the digestive ability of termites on wood depends mainly on the intestinal microbes in some literatures. In order to know the microbes in the gut of termites, in this experiment, by regarding Odontotermes formosanus as materials, 112 species were separated and purified from the gut of termites, and 35 strains are aerophile and 77 strains are anaerobic. In all microorganisms, 12 aerophile species, which may have the activity of cellulose, were screened by Congo red method initially. the largest transparent circle get up to 4.0 ㎝×4.0 ㎝. Further, the cellulase activities of 12 strains were checked. The results