大鼠肌内注射20_S_人参皂苷Rg3的药动学研究.pdf

20(S )- Rg3 1 2 2 2 * 刘继华, 卢丹, 刘金平,李平亚 (1., 130021;2., 130021) :　研究 20(S )-人参皂苷 Rg3经肌内注射后在大鼠体内的药动学情况, 括血药动力学研究, 组织分布情况,在尿和 胆汁中的排泄情况等方面的内容。　本实验采用蒸发光散射检测法测定药物在血液和组织中的浓度, 用 3P97药动学程 序进行数据处理, 方法快捷、简便, 结果的精密度、稳定性和回收率均好。　20(S )-人参皂苷 Rg3肌内注射后(1.5 mg - 1 - 1 - 1 kg ), 其药动学模型为一室模型, t 为 0.75 h, t 为 0.116 h, ρ为 13.9 mg L , AUC为 16.6 mg h L ;肌注后在 1 /2(ke) (eak ) m ax 心、肝、脾、肺、肾中可以检测到有 20(S )-人参皂苷 Rg3存在, 而在脑、胃、肠、体脂、肌肉和生殖腺中均未检测到;药物主要经尿 液排泄, 给药 8 h 内, 20(S)-人参皂苷 Rg3经尿液排出药物总量的 72.1%, 经胆汁排泄的药物占药物总量的 10.1%;药物与蛋 白的结合率为 15%～ 17%, 且不随浓度的变化而改变。　20(S )-人参皂苷 Rg3肌内注射后可以很快被吸收进入血液, 迅 速达到血药浓度的峰值, 它在体内的代谢速度较快, 且大部分药物经尿液由肾脏排出体外,在体内基本无蓄积。 :20(S )-人参皂苷 Rg3;药动学;蒸发光散射检测法 :R969　　　:A　　　:1001- 2494(2007)14 - 1087 - 04 Studies on Pharmacokinetics of 20(S)-GinsenosideRg3 A fter IntramuscularAdm inistration toRats 1 2 2 2*(1.Phamarcy Institute, J ilin Universiyt, Changchun 130021, China; LIU J-i hua, LU Dan, LIU Jin-ing, LI Ping-ya 2.Institute of F ron tierMed icine of J ilin Universiyt, Changchun 130021, China) ABSTRACT:OBJ CTIV 　To investigate the harm acokinetics of 20(S)-ginsenoside Rg3 in lasm a, d istribution in organs, and e-x cretion by u rine and b ile after intram us cu lar adm in istration to rats.M THODS　Rat lasma sam lesw ere rocessed by solid hase e-x traction and the concentrationswere detem ined by HPLC /ELSD(Eva orative Ligh-t scattering Detector)method.The rogram of 3P97 was a lied to rocess the concentration-tmi e curves of 20(S)-ginsenoside Rg3 in rat lasm a after in tramuscular injection(mi )of 1.5 m g kg- 1 .R SULTS　The harm acok inetics of 20(S )-ginsenoside Rg3 follow ed first order kinetics and lasma concentration versus tmi e data were adequately described by an one-com artment m odel.The c