椪柑原生质体分离与培养过程中活性氧动态变化及来源分析.pdf

Botanical Research 植物学研究, 2016, 5(3), 67-73 Published Online May 2016 in Hans. /journal/br /10.12677/br.2016.53010 The Dynamic Changes and Origin Analysis of Reactive Oxygen Species during Isolation and Culture of Citrus reticulata Blanco Protoplasts Xiaoyong Xu, Yihui Zhang, Yuping Ling School of Horticulture and Plant Protection, Yangzhou University, Yangzhou Jiangsu rd th th Received: May. 3 , 2016; accepted: May. 20 , 2016; published: May. 30 , 2016 Copyright © 2016 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract In order to uncover the mechanisms underlying different regeneration capacity of protoplasts, we analyzed the dynamic changes and origin of reactive oxygen species (ROS) during isolation and culture of Citrus reticulata Blanco protoplasts. The dynamic changes of ROS were analyzed by flu- orescent markers, and ROS origin was investigated by ROS indicators. The results showed that ROS signals were visible in the cytoplasm and cell membrane of protoplasts; compared with me- sophyll protoplasts, callus protoplasts contained significantly lower level of ROS during isolation, but significantly higher level of ROS during culture; a significant decrease in the level of ROS dur- ing isolation and culture of callus protoplasts was observed after DPI or NaN3 treatment. Our findings indicate ROS plays a crucial mediating role in protoplast regeneration of Citrus reticulata. Keywords Protoplast, Reactive Oxygen Species, DPI, NaN3 椪柑原生质体分离与培养过程中活性氧动态变 化及来源分析 徐小勇，张一卉，凌裕平 文章引用: 徐小勇, 张一卉, 凌裕平. 椪柑原生质体