玫瑰干花精油提取方法比较研究.PDF

玫瑰干花精油提取方法比较研究 毛佩芝1 2* 2 ，王龙虎 ，雷高明 (1.宁波市妇女儿童医院，浙江 宁波 315000 ； 2.浙江大学药学院，杭州 310058) 摘要：目的 对比研究玫瑰精油3 种提取方法，探讨所得玫瑰干花精油化学成分的变化。方法 料液比为 1 ∶8，加5%氯 化钠溶液静置4 h ，蒸馏时间为3 h ，分别用蒸馏萃取同步法、溶剂萃取分离法、二次蒸馏法得到玫瑰干花精油，应用GC-MS 定性分析其化学成分。结果 蒸馏萃取同步法和二次蒸馏法各鉴定出22 种化合物，溶剂萃取分离法鉴定出5 种化合物， 三者的化学成分中既有共同组分，也存在一定的差异性。结论 先加正己烷的蒸馏萃取同步法可以克服油水分离困难， 有利于提升水蒸气法的精油回收。 关键词：玫瑰干花；水蒸气蒸馏法；气相色谱-质谱法；玫瑰精油 中图分类号：R284.2 文献标志码：B 文章编号：1007-7693(2017)04-0538-04 DOI: 10.13748/ki.issn1007-7693.2017.04.014  Comparative Study of Extraction Methods of Rose Flower Essential Oil 1 2* 2 MAO Peizhi , WANG Longhu , LEI Gaoming (1.Ningbo Women Children’s Hospital, Ningbo 315000, China; 2.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China) ABSTRACT: OBJECTIVE To study on three methods for extraction of rose flower essential oil, to investigate the changes of the chemical constituents. METHODS The conditions were: solid to liquid ratio 1 ∶8, adding 5% concentration of sodium chloride and standing for 4 h, distillation for 3 h. Rose essential oil were isolated using simultaneous distillation and extraction method, solvent extraction separation method and secondary distillation method, respectively. The chemical compositions were analyzed using GC-MS. RESULTS The 22 components were identified by simultaneous distillation and extraction method and secondary distillation method, respectively; 5 components were identified by solvent extraction separation method, the essential oils obtained by three methods contained common components and also showed differences. CONCLUSION The method of adding hexane before distillation can overcome the difficulty of oil-water separation, which is beneficial to the recovery of oil via steam distillation. KEY