Bt棉棉子中Bt蛋白定量检测方法建立及变化规律研究_徐宝梁.pdfVIP

Cotton Science 2006, 18( 1) : 19~ 22 t 棉棉子中 t 蛋白定量检测方法建立及变化规律研究 1, 2 2 1 1 2 2 1* , , , , , , ( 1. 中国农业大学, 北京100061; 2. 中国检验检疫科学研究院, 北京100025) : 应用Envirologix 试剂盒测定99 33 棉子仁中 t 蛋白的含量, 分 为4376.4 ng # g-1 -1 和4321.7 ng # g ; 测定系列稀释的棉子仁提取液, Envirologix 试剂盒测得的OD450 和Agdia 试剂盒测得的OD630 间的相关性很好, 相关系数接近0.99; 而Agdia 试剂盒测得的系列稀释液 的OD630 与其 t 蛋白含量间有很好的相关性, 相关系数大于0. 99据此, 利用Agdia 试剂盒 测得99 33 棉子棉子仁棉子壳在 12 个月的贮存过程中 t 蛋白含量的变化规律, 棉子 棉子仁中 t 蛋白含量降低一半, 棉子壳中 t 蛋白含量降低四分之一 : t 棉棉子; t 蛋白含量; 变化规律 :S433. 1 :A : 1002-7807(2006) 01-0019-04 Study on the Quantitative Method and the Degradation Rule of Bt protein in Bt Cottonseeds 1, 2 2 2 1 2 2 XU ao-liang , SU Ning , YUAN Fei , WANG Yin-xia , CHEN Ying , WU Ya-jun , ZHANG Qing-wen1* ( 1. Chi na A g r icul t ur al Un iv ers i ty , Beij ing 100061, Ch ina; 2. Chi nese A c ademy of I nsp e cti on and Quar anti ne, Be ij ing 100025, Ch i na) Abstract: Cottonseeds, cottonseed kernels and cottonseed shells of 99 and 33 were stored in a dry and dark place at room temperature. Samples were taken every month and grounded. Extractions of cottonseeds, kernels and shells were prepared using the method described in the instructions of the kits. T he extractions were serially diluted with the extraction buffers at the ratio of 1B 1 ( volume to volume) . t protein contents of cottonseed kernels were determined using Envirologix kit followed the instruction of the kit. Optical densities of the extraction and the diluted extractions from cottonseeds, kernels and shells at 450 nm and 630 nm were determined using Envirologix kit and Agdia kit respec- tively followed the instructions of