蒋玉蓉小论文.doc

可溶性Endoglin与子痫前期发病关系的研究 蒋玉蓉 张丽娟 【摘 要】 目的 探讨可溶性endoglin(Soluble endoglin sEng)与子痫前期发病的关系。方法 (1)采用酶联免疫吸附试验(ELISA)测定30例子痫前期患者(子痫前期组)分娩前及分娩后血清sEng水平，其中轻度子痫前期14例，重度子痫前期16例。并以30例健康孕妇作为对照(对照组)，测定其早期妊娠、中期妊娠、晚期妊娠血清sEng水平。(2)采用蛋白印迹法（Western Blot)检测各组孕妇胎盘组织中sEng蛋白的表达水平。结果（1）sEng在正常早、中、晚孕组血清中表达水平分别为1.21±0.23ng/ml、1.78±0.28ng/ml、2.85±0.38ng/ml，中孕组和晚孕组高于早孕组，差异有统计学意义(P0.05)，晚孕组高于中孕组，差异有统计学意义(P0.05)。（2）正常妊娠产后组sEng水平(2.06±0.30ng/ml)均较晚期妊娠妇女减低，差异有显著性(P0.01)。（3）sEng在子痫前期为6.17±1.41ng/ml，与对照组晚期妊娠妇女比较，差异均有显著性(P0.01)。子痫前期重度组（7.33±0.77ng/ml）高于子痫前期轻度组（4.86±0.51ng/ml），差异均有统计学意义(P0.01)。（4）子痫前期产后组sEng水平(2.19±0.29ng/ml)与正常妊娠产后组比较差异无统计学意义(P0.05)。（5）妊娠34周前发病子痫前期患者血清sEng水平分别为7.96±0.64ng/ml，较妊娠34周后发病的子痫前期患者血清sEng水平(6.82±0.37ng/ml)明显升高(P0.01)。（6）sEng蛋白在重度子痫前期组、轻度子痫组胎盘组织中的表达为0.60±0.14、0.32±0.11，明显高于对照组晚期妊娠妇女0.16±0.02，差异有统计学意义(P0.01)，且子痫前期组重度患者高于子痫前期组轻度患者(P0.01)。（7）对照组血清sEng水平与胎盘sEng蛋白表达水平有相关性(r=0.523；P0.05);子痫前期组血清sEng水平与胎盘sEng蛋白表达水平也有相关性(r =0.836；P0.01)。结论 （1）子痫前期患者血清中sEng水平升高并与其病情严重程度相关。（2）子痫前期患者通过胎盘组织中胎盘sEng蛋白过度表达，引起血循环中sEng水平升高，参与子痫前期的病理生理过程。 【关键词】 子痫前期；endoglin；可溶性endoglin；蛋白印迹 作者单位：410011，长沙，中南大学湘雅二医院妇产科 通讯作者：张丽娟，Email:zlj621116@ The study of the relationship between the soluble endoglin and preeclampsia JIANG Yu-rong ZHANG Li-juan ,Department of Gynecology and Obstetrics，Xiangya Second Hospital，Central South University，Changsha 410011，China Corresponding author: ZHANG Li-juan, E-mail: zlj621116@ 【Abstract】Objective:Tinvestigate the relationship bewteen soluble endoglin(sEng) and preeclampsia. Methods:The levels of sEng in seurm of 30 women with preeclampsia(including mild preeclampsia 14 cases, severe preeclampsia 16 cases )and 30 nomral Prengnat women (including first trimester pregnancy,2nd trimester pregnancy,and 3rd trimester pregnancy) were detected by enzyme linked immuosorbent assay.Western blot was used to investigate the expression of sEng in placentas of these women.Results:(1) The seurm level of sEng of the third trimester pregnancy(2.85±0.38ng