具有细菌群体感应抑制活性海洋来源真菌的筛选鉴定.PDF

生 物 工 程 学 报 Chin J Biotech 2011, September 25; 27(9): 1337−1346 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2011 CJB, All rights reserved. 海洋生物技术 具有细菌群体感应抑制活性海洋来源真菌的筛选鉴定 尹守亮，常亚婧，邓苏萍，王清池，于文功，宫倩红 中国海洋大学医药学院 海洋药物教育部重点实验室，青岛 266003 摘 要: 许多致病菌的致病机制依赖于群体感应系统的调控，经实验证明群体感应系统突变或缺失的菌株致病能力显 著下降，筛选高效的群体感应抑制剂有望成为解决细菌感染以及细菌耐药性问题的一个有效途径。从海洋软体动物体 内分离海洋真菌69 株，发酵液粗提物经 QSIS2 (Quorum Sensing Inhibitor Selector 2) 筛选模型和紫色杆菌CV026 指示 菌株筛选后得到编号QY013 的粗提物具有群体感应抑制活性，进一步实验表明该粗提物能够显著降低铜绿假单胞菌群 体感应调控的毒力因子绿脓菌素的产量，以及紫色杆菌群体感应调控的紫色菌素的产量，且在有效浓度范围内对细菌 生长不产生影响。形态学特征和 18S rDNA 序列分析表明菌株 QY013 为Penicillium 属。文中筛选到一株具有细菌群体 感应抑制活性的海洋来源真菌，其发酵液粗提物中的有效活性成分可用于新型抗菌药物的研究。 关键词: 群体感应，海洋真菌，QSIS2 ，紫色杆菌CV026 ，18S rDNA Screening and identification of marine fungi against bacterial quorum sensing Shouliang Yin, Yajing Chang, Suping Deng, Qingchi Wang, Wengong Yu, and Qianhong Gong Key Laboratory of Marine Drugs , Ministry of Education, College of Medicine and Pharmaceutics, Ocean University of China , Qingdao 266003, China Abstract: The discovery of quorum sensing (QS) system and its critical role in bacterial virulence have revealed a new way to attack pathogenic bacterium. The pathogenecity of QS deletion mutants decreases significantly. Targeting bacterial QS system is a promising therapeutic approach to control infections and anti-microbial resistance. To obtain natural QS inhibitors from marine organisms, marine fungi (69 strains) were isolated from marine mollusca, and their extracts were screened using improved QSIS2 (Quorum Sensing Inhibitor Selector 2) assay and Chromobacterium violaceum CV026. To improve the efficiency