新型荧光金纳米粒子的制备及Cu2+检测.PDF

安徽农业大学学报, 2013, 40(1): 155-158 Journal of Anhui Agricultural University 网络出版时间：2012-12-28 13:30:22 [URL] /kcms/detail/34.1162.S1330.017.html 新型荧光金纳米粒子的制备及 Cu2+检测 汪松美，宋晓朋 (江苏大学环境学院，镇江 212013) 摘 要：在水溶液中以 L- 半胱氨酸（L-Cys ）为还原剂和稳定剂制备了具有一定荧光性能的金纳米团簇 （L-Cys-AuNCs ），并对其结构及荧光性能进行表征。研究发现Cu2+对 L-Cys-AuNCs 的荧光强度有特殊的响应，基 于此建立了一种快捷方便的痕量铜离子的检测方法。考察了 L-Cys 与金盐摩尔比、溶液 pH 、反应时间和温度等因 素对测定的影响。结果表明，在最优实验条件下，L-Cys-AuNCs 的荧光强度与 Cu2+ 的浓度呈良好的线性关系，当 Cu2+ -6 -4 -1 2+ 浓度范围为 10 ～10 mol·L 时，L-Cys-AuNCs 的荧光强度与 Cu 浓度呈良好的线性关系，线性方程为 F = －67.541c + 703.02 （R=0.995 ）。该方法成功地应用于水样中铜离子的检测。 关键词：金纳米团簇（L-Cys-AuNCs ）；荧光猝灭；Cu2+ 中图分类号：Q657.3 文献标识码：A 文章编号：1672−352X (2013)01−0155−04 2+ Preparation of novel fluorescent Au nanoparticles and its application for Cu assay WANG Song-mei, Song Xiao-peng (School of the Environment, Jiangsu University, Zhenjiang 212013) Abstract: In this paper, a series of fluorescent gold nanoclusters (L-Cys-AuNCs) were prepared using L-cysteine as capped agent. The fluorescence and structure of the prepared L-Cys-AuNCs were also character- ized. It was found that the fluorescence intensity of L-Cys-AuNCs could be quenched sensitively in the pres- 2+ ence of Cu ions. Therefore, the AuNCs based fluorescent probe was reported for the rapid and selective detec- 2+ 2+ tion of Cu ions. Potential factors affecting the performance of the probe for the detection of Cu ions were carefully optimized, includi