Effusion cytology quality control.doc

Effusion cytology quality control [Keywords:] tumor; cytological techniques; effusion Effusion often associated symptoms of certain diseases, as initial symptoms or the emergence of clinical examination often through identification of serous effusions of benign and malignant effusion, a clear cause. At present, the serous effusion cytology found in tumor cells is still the identification of benign and malignant serous effusions is a fast, accurate, reliable and economical method of choice. centrifugation method for routine detection of tumor cells positive rate was 33% ~ 60% [12], So many years, effusion cytology method continuous innovation and strengthen quality control to improve various aspects of the detection rate of tumor cells by more and more attention to the clinical laboratory. An analysis of quality control before 1.1 Specimen Collection 1.1.1 Collection methods on large effusion should be sacrificing the final out, with more cells in serous cavity fluid off to do cytology. Xueli Fu introduced [3], in the pleural effusion can be collected in fluid drawn into the syringe first and then rapidly brought to the bottom of the chest, repeated several times collected for inspection, make tumor cells to improve the detection rate from 50% to 80.3%. 1.1.2 anticoagulant cytology specimens should be collected by adding an anticoagulant, usually heparin 300U/100mL mostly specimens, or 109mmol / L sodium citrate (samples with anticoagulant ratio of 10:1) is improve the detection rate of tumor cells is an important part and can not be ignored. 1.2 for inspection 1.2.1 the amount of sample submission is directly related to the amount of inspection samples can be collected from the amount of tumor cells, most investigators recommended 200mL or so. Zhizhong et al [4] to 116 cases of pleural effusion specimens were divided into 100 ~ 300mL group and 5 ~ 10mL group to do comparison, the results of tumor cell detection rate was 83.6% and 46.6%, the d