Evodia Extract Antioxidant.doc

Evodia Extract Antioxidant Of: climb Shiguo Ru Zhang Wei Qiao Haixia Chen [Abstract] Objective: To study the scavenging Evodia Extract ( OH) and superoxide anion radical ( O-2) effect. Methods: ferrous sulfate and hydrogen peroxide reaction OH; with o-phenylenediamine Autoxidation method produces O-2. spectrophotometric determination of butanol extract of Evodia 1, butanol extract and ether extract 2 and O-2 on OH scavenging. Results: Three clear extract and Vc OH, IC50 145.0,141.0,335.0,2.3 g mL-1; butanol extract a clear role of O-2 can not be measured, n-butanol extract of Evodia 2, ether extract Vc clear material and O-2, IC50 270.0,0.185,0.057 5g mL-1. Conclusion: This study had three Evodia extract certain antioxidant and a dose relationship. Evodia soluble components clear the role of O-2 was significant, with similar effects Vc. [Keywords:] Evodia; antioxidant; hydroxyl radical; superoxide anion radical [ABSTRACT] Objective: To study the removal function of exfract from Evodia rutaecarpa (Juss.) on hydroxyl radical ( OH) and Superoxide anion radical ( O-2). Methods: Kreaction of ferrous sulfate and hydrogen peroxide was used to generate OH, with the pyrogallol autoxidation method producing O-2.The removing ability of n butanol extract 1, n butanol extract 2 and ether extract was determined by spectrophotometry.Results: IC50 of scavenging OH for these three kinds of extracts and VC were 145.0,141.0,335.0,2.3 g mL-1 respectively; but the ability of removing O-2 of n butanol extract 1 could not be determined, IC50 of the rest three were 270.0,0.185 and 0.057 5g mL-1 respectively.Conclusion: Three extracts from Evodia rutaecarpa (Juss.) Benth. have some anti oxidation, and showed dose effect relationship.The ability of removing O-2 for liposolubles was very remarkable, and it had similar ability with that of Vc. [KEY WORDS] Evodia rutaecarpa (Juss.) Benth., Antioxidation, Hydroxyl radicals, Superoxide anion radical Free radicals are mo