1672基因敲除显著影响肺炎链球菌的毒力spd1672.PDF

J South Med Univ, 2015, 35(8): 1197-1200 doi 10.3969/j.issn. 1673-4254.2015.08.23 ·1197 · 基础研究 spdsp基基因敲除显著影响肺炎链球菌的毒力因敲除显著影响肺炎链球菌的毒力 1 2 3 1 1 1 黄 健 ，黄美容 ，吴凯峰 ，朱杰华 ，黎 兵 ，闵 迅 1 2 3 遵义医学院附属医院 检验科，输血科，贵州 遵义 563003 ；遵义市第一人民医院检验科，贵州 遵义 563002 摘要：目的研究spd1672基因在肺炎链球菌感染过程中的作用。方法 通过腹腔攻毒实验和细菌入血载量分析、细菌的粘附侵 袭实验、全血杀菌实验以及相关细胞因子检测等观察spd1672基因敲除（D39 △1672菌株）后对肺炎链球菌毒力的影响。结果 D39 △1672菌株感染组小鼠中位生存时间和生存率显著高于野生菌株组（P0.05），而血中细菌载量则显著低于野生菌株感染 组（P0.05）。与野生菌株比较，D39 △1672菌株对A549 细胞的粘附能力无明显差异，但其侵袭能力显著受损（P0.05）。此外， D39 △1672菌株对全血杀菌作用的抵抗能力亦较野生菌显著减弱（P0.05），小鼠体内炎性细胞因子水平显著降低。结论 spd1672基因与肺炎链球菌的侵袭、抗宿主能力和致炎反应等过程相关。 关键词：肺炎链球菌；spd1672基因；毒力因子 Spd1672 gene knockout significantly attenuates the virulence of Streptococcus pneumoniae 1 2 3 1 1 1 HUANG Jian , HUANG Meirong , WU Kaifeng , ZHU Jiehua , LI Bing , MIN Xun 1Department of Clinical Laboratory, 2Department of Blood Transfusion, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China; 3Department of Clinical Laboratory, First Peoples Hospital of Zunyi, Zunyi 563002, China Abstract: Objective To investigate the role of spd1672 gene in the infection process of Streptococcus pneumoniae. Methods BALB/ c mice were intraperitoneally infected by a spd1672 knockout strain and a D39 wild-type strain of S. pneumoniae, and the survival time of mice and blood bacterial counts were recorded. The adhesion and invasion ability of S. pneumoniae strains were assessed in A549 cells. Bactericidal assays were carried out to determine the resistance of spd1672 knockout strains and D39 wild strains, and the serum levels of inflammatory cytokines were detected in the infected mice. Results The mice infected with spd1672 knockout strains