一株产绿色素放线菌的初步鉴定及其色素稳定性的检测.pdfVIP

第 46 卷 2010 年第 3 期 西北师范大学学报(自然科学版) VoL 46 2010 No.3 Journal of Northwest Normal University (Natural Science) 89 一株产绿色素放线菌的初步鉴定 及其色素稳定性的检测 张爱祷，牛世全，达文燕，虎坞，苏 雪，姚雪茹，药、坤餐 (西北师范大学生命科学学院，甘肃兰州 730070) 摘 要:从天就县狐喜秀龙乡境内采集的西藏沙幸草根董事土埃中分离得到一株产绿色素放线篱，初步鉴定其为链霉蔼属 绿色类群链霉菌，其所产色素为一种水溶位色素，该色素在低浓度日202 存在对稳定性较好，萄萄糖浓度越高，色素 约稳定性越差I j是糖的存在可以增加色素约色度，低浓度的 NaCI 能使色素绿色加深，而色素在高浓度NaCI 存在时比 较稳定，还原剂豆就酸钱对色素有增色作用且比绞稳定.色素在中a注或革良性条件下绿色加漾，长对间放至后元，大的变 化，色素在 40-60 c能够较长时间保持色度不变.金属离子 Na+ 、 M~夺、 Ca2+和 FeZ+ 在短时何内毒草增加色素的色 皮，豆色素在 MnZ+存在对性凌比较稳定. 关键词:放线蔼s 鉴定;微生物色素z 色素稳定a性 中图分类号: Q 93-331 文献标识码:A 文章编号: 1001-988X (2010)03-0089-05 Identification of an actinomyces producing green pigment and detection of pigment stability ZHANG Ai-mei , NIU Shi-quan , DA Wen-yan , HU Rui , SU Xue , YAO Xue--r毡， SUN Kun (College of Life Sciences , Northwest Normal University , Lanzho延 730070 ， Gansu , China) Abslract: A strain Streptomyce actinomyces of producing green pigment is obtained from rhizosphere soil of Hippoρhae thibetana. The pigment can dissolve in water.τhe pigment is stable when H 2 0 2 is low concentration where it is not stable when the glucose concentration is higher. The pigment will become greener when there is sucrose , NaZ S03 and low concentration NaCI , and also become greener in the neutral or alkaline solution too.τhe pigment can retain green when the NaCI concentration is higher and the temperature is between 40 c and 60 C. The color of the pigment will become greener when adding 2 2 Na+ , Mg + , Ca + and Fe2+ in solution in shorter time , while the pigment is stable when there is MnZ+. Key words: actinomyces; identification; green pigment; pigment stability 天然色素因其安全无毒，并亘具有特有的营养